Inhibition of epidermal growth factor-induced mitogenesis by amiloride and an analog: Evidence against a requirement for Na super(+)/H super(+) exchange

The authors have tested the hypothesis that the rapid stimulation of Na super(+)/H super(+) exchange by epidermal growth factor (EGF) is a requirement for induction of mitogenesis. The authors conclude that (i) inhibition of EGF-induced mitogenesis by amiloride is due not to inhibition of EGF-stimul...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (21), p.6762-6766
Main Authors: Besterman, J M, Tyrey, S J, Cragoe, EJ Jr, Cuatrecasas, P
Format: Article
Language:English
Subjects:
3T3 cells
epidermal growth factor
ion exchange
mitogenicity
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Summary:The authors have tested the hypothesis that the rapid stimulation of Na super(+)/H super(+) exchange by epidermal growth factor (EGF) is a requirement for induction of mitogenesis. The authors conclude that (i) inhibition of EGF-induced mitogenesis by amiloride is due not to inhibition of EGF-stimulated Na super(+)/H super(+) exchange but rather to inhibition of necessary events occurring during the hours immediately prior to the onset of DNA synthesis, these events probably being RNA and protein synthesis and (ii) in cell culture medium buffered with CO sub(2)/HCO sub(3) super(-), complete inhibition of EGF-stimulated Na super(+)/H super(+) exchange does not inhibit EGF-induced mitogenesis and, thus, stimulation of Na super(+)/H super(+) exchange is not necessary for induction of mitogenesis by EGF.
ISSN:0027-8424