Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease

Bacterial endophytes offer control against many diseases of crop plants as potential biocontrol agents. Antagonistic bacterial endophytes acting against Phytophthora meadii have been screened from leaf, petiole and root tissues of Hevea brasiliensis. Six bacterial endophytes could exhibit more than...

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Bibliographic Details
Published in:BioControl (Dordrecht, Netherlands) Netherlands), 2013-10, Vol.58 (5), p.675-684
Main Authors: Abraham, Amith, Philip, Shaji, Kuruvilla Jacob, C, Jayachandran, K
Format: Article
Language:English
Subjects:
Agriculture
Alcaligenes
Animal Biochemistry
Animal Ecology
Antibiotics
Bacteria
Behavioral Sciences
Bioassays
Biological control
biological control agents
Biomedical and Life Sciences
clones
Cloning
crops
Culture techniques
Disease control
Endophytes
Entomology
Genetic diversity
genetic variation
Hevea brasiliensis
Leaf fall
Leaves
Life Sciences
nucleotide sequences
Pathogens
petioles
Phytophthora
Phytophthora meadii
Plant diseases
Plant Pathology
Plant resistance
Plant tissues
Pontella meadii
Pseudomonas aeruginosa
Reinforced reaction injection molding
Ribosomal DNA
rRNA 16S
Sequence analysis
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Summary:Bacterial endophytes offer control against many diseases of crop plants as potential biocontrol agents. Antagonistic bacterial endophytes acting against Phytophthora meadii have been screened from leaf, petiole and root tissues of Hevea brasiliensis. Six bacterial endophytes could exhibit more than 50 % inhibition of P. meadii, among which EIL-2, from disease-free zones showed a maximum of 62.5 % inhibition. The isolate EIL-2 was characterized as Alcaligenes sp. and the other isolates were identified as Pseudomonas aeruginosa. 16S rDNA sequence analysis showed that there existed genetic variation among the five isolates of P. aeruginosa from different tissues of the plant indicating the tissue type adaptation of the isolates. Dual culture technique with endophyte EIL-2 completely arrested the growth of P. meadii when inoculated prior to pathogen. The bioassay with EIL-2 in H. brasiliensis clones, RRII 105 showed 43 % reduction of lesion size on infected leaves whereas in RRIM 600 it was only 30 %.
ISSN:1386-6141
1573-8248
DOI:10.1007/s10526-013-9516-0