Isocitric acid production from rapeseed oil by Yarrowia lipolytica yeast

Production of d S - threo -isocitric acid (ICA) by yeast meets serious difficulties since it is accompanied by a simultaneous production of citric acid (CA) in significant amounts that reduces the yield of desired product. In order to develop an effective process of ICA production, 60 yeast strains...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2013-10, Vol.97 (20), p.9133-9144
Main Authors: Kamzolova, Svetlana V., Dedyukhina, Emiliya G., Samoilenko, Vladimir A., Lunina, Julia N., Puntus, Irina F., Allayarov, Ramil L., Chiglintseva, Maria N., Mironov, Alexey A., Morgunov, Igor G.
Format: Article
Language:English
Subjects:
Acid production
Aeration
Agricultural biotechnology
Applied Microbial and Cell Physiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Candida
Canola Oil
Carbon
Citric acid
Culture Media - metabolism
Dehydrogenases
Diastereomers
Fatty acids
Fatty Acids, Monounsaturated
Isocitrates - metabolism
Life Sciences
Microbial Genetics and Genomics
Microbiology
Microorganisms
Natural products
Physiology
Pichia
Plant Oils - metabolism
Rapeseed oil
Saccharomyces
Torulopsis
Trace elements
Yarrowia
Yarrowia - metabolism
Yarrowia lipolytica
Yeast
Yeasts
Yeasts - metabolism
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Summary:Production of d S - threo -isocitric acid (ICA) by yeast meets serious difficulties since it is accompanied by a simultaneous production of citric acid (CA) in significant amounts that reduces the yield of desired product. In order to develop an effective process of ICA production, 60 yeast strains of different genera ( Candida , Pichia , Saccharomyces , Torulopsis , and Yarrowia ) were tested for their ability to produce ICA from rapeseed oil; as a result, wild-type strain Yarrowia lipolytica VKM Y-2373 and its mutant Y. lipolytica 704-UV4-A/NG50 were selected as promising ICA producers. The effects of temperature, pH, aeration, and concentrations of rapeseed oil, iron, and itaconic acid on ICA production by selected strains were studied. Under optimal conditions (pH 6.0; aeration 50–55 %; rapeseed oil concentration of 20–60 gl −1 , iron ion concentration of 1.2 mg l −1 , and itaconic acid amount of 30 mM), selected strains of Y. lipolytica produced predominantly ICA with a low amount of a by-product, CA.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-013-5182-5