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Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells
Control of staphylococci during cleaning and disinfection is important to the food industry. Broad-spectrum bacteriocins with proved anti-staphylococcal activity, such as enterocin AS-48, could open new possibilities for disinfection in combination with biocides. In the present study, enterocin AS-4...
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| Published in: | International journal of food microbiology 2013-05, Vol.163 (2-3), p.96-100 |
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| cited_by | cdi_FETCH-LOGICAL-c464t-8783b1e8724ec6eed2e0532a593a1ddc334be532b25234db98ff57586fc64dc93 |
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| cites | cdi_FETCH-LOGICAL-c464t-8783b1e8724ec6eed2e0532a593a1ddc334be532b25234db98ff57586fc64dc93 |
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| container_start_page | 96 |
| container_title | International journal of food microbiology |
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| creator | Caballero Gómez, Natacha Abriouel, Hikmate Grande, Mª. José Pérez Pulido, Rubén Gálvez, Antonio |
| description | Control of staphylococci during cleaning and disinfection is important to the food industry. Broad-spectrum bacteriocins with proved anti-staphylococcal activity, such as enterocin AS-48, could open new possibilities for disinfection in combination with biocides. In the present study, enterocin AS-48 was tested singly or in combination with biocides against a cocktail of six Staphylococcus aureus strains (including three methicillin-resistant strains) in planktonic state as well as in biofilms formed on polystyrene microtiter plates. Cells were challenged with enterocin, biocides or enterocin/biocide combinations. Inactivation of planktonic cells increased significantly (p |
| doi_str_mv | 10.1016/j.ijfoodmicro.2013.02.018 |
| format | article |
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•A cocktail of methicillin resistant and sensitive staphylococci was sensitive to biocides and enterocin AS-48.•Combined treatments of enterocin/biocide improved inactivation of planktonic and sessile staphylococci.•The combined treatments delayed or avoided recovery of treated biofilms during storage.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2013.02.018</identifier><identifier>PMID: 23558192</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Bacteriocin ; bacteriocins ; Bacteriocins - pharmacology ; benzalkonium chloride ; Biocides ; Biofilm ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; bromides ; cell growth ; chlorhexidine ; chlorides ; cleaning ; Disinfectants - pharmacology ; disinfection ; Disinfection - standards ; Food industries ; food industry ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; hexachlorophene ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - physiology ; Microbial Viability - drug effects ; Plankton - drug effects ; polystyrenes ; Staphylococcus ; Staphylococcus aureus ; Time Factors</subject><ispartof>International journal of food microbiology, 2013-05, Vol.163 (2-3), p.96-100</ispartof><rights>2013 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-8783b1e8724ec6eed2e0532a593a1ddc334be532b25234db98ff57586fc64dc93</citedby><cites>FETCH-LOGICAL-c464t-8783b1e8724ec6eed2e0532a593a1ddc334be532b25234db98ff57586fc64dc93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27369370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23558192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caballero Gómez, Natacha</creatorcontrib><creatorcontrib>Abriouel, Hikmate</creatorcontrib><creatorcontrib>Grande, Mª. José</creatorcontrib><creatorcontrib>Pérez Pulido, Rubén</creatorcontrib><creatorcontrib>Gálvez, Antonio</creatorcontrib><title>Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Control of staphylococci during cleaning and disinfection is important to the food industry. Broad-spectrum bacteriocins with proved anti-staphylococcal activity, such as enterocin AS-48, could open new possibilities for disinfection in combination with biocides. In the present study, enterocin AS-48 was tested singly or in combination with biocides against a cocktail of six Staphylococcus aureus strains (including three methicillin-resistant strains) in planktonic state as well as in biofilms formed on polystyrene microtiter plates. Cells were challenged with enterocin, biocides or enterocin/biocide combinations. Inactivation of planktonic cells increased significantly (p<0.05) when enterocin AS-48 (25mg/l) was tested in combination with benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and non-significantly in combination with didecyldimethylammonium bromide (AB), triclosan (TC), hexachlorophene (CF), polyhexamethylen guanidinium chloride (PHMG), chlorhexidine (CH) or P3-oxonia (OX). In the sessile state (24h biofilms), staphylococci required higher biocide concentrations in most cases, except for OX. Inactivation of sessile staphylococci increased remarkably when biocides were applied in combination with enterocin AS-48, especially when the bacteriocin was added at 50mg/l. During storage, the concentrations of sessile as well as planktonic cells in the treated samples decreased remarkably for BC, TC and PHMG, but OX failed to inhibit proliferation of the treated biofilms as well as growth of planktonic cells. The observed inhibitory effects during storage were potentiated when the biocides were combined with 50mg/l enterocin AS-48. Results from this study suggest that selected combinations of enterocin AS-48 and biocides offer potential use against planktonic and sessile, methicillin-sensitive and methicillin-resistant S. aureus.
•A cocktail of methicillin resistant and sensitive staphylococci was sensitive to biocides and enterocin AS-48.•Combined treatments of enterocin/biocide improved inactivation of planktonic and sessile staphylococci.•The combined treatments delayed or avoided recovery of treated biofilms during storage.</description><subject>Bacteriocin</subject><subject>bacteriocins</subject><subject>Bacteriocins - pharmacology</subject><subject>benzalkonium chloride</subject><subject>Biocides</subject><subject>Biofilm</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>bromides</subject><subject>cell growth</subject><subject>chlorhexidine</subject><subject>chlorides</subject><subject>cleaning</subject><subject>Disinfectants - pharmacology</subject><subject>disinfection</subject><subject>Disinfection - standards</subject><subject>Food industries</subject><subject>food industry</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hexachlorophene</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - physiology</subject><subject>Microbial Viability - drug effects</subject><subject>Plankton - drug effects</subject><subject>polystyrenes</subject><subject>Staphylococcus</subject><subject>Staphylococcus aureus</subject><subject>Time Factors</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkcuO0zAUhiMEYsrAK4BZILFJ8S2JsxxVDCCNxKLM2nLsE3pKYhfbLZoX4_nGpeW2gtWRzvn-c_ur6iWjS0ZZ-2a7xO0YgpvRxrDklIkl5UvK1INqwVTX10K29GG1KKyqWUubi-pJSltKaSMEfVxdcNE0ivV8UX1fhXlAD47kCCbP4HMiYSQlQgwWPbla11KRb5g3ZMCScZBIDgTnXQwHIHkDBL2xGQ8mY_BH8Qx5gxanCX2dwCcsRSDGu78qERKmbHwm62x2m7sp2GDtPhGzj1DCbjL-Sw4e7Q9pgpRwAmJhmtLT6tFopgTPzvGyur1--2n1vr75-O7D6uqmtrKVuVadEgMD1XEJtgVwHMoLuGl6YZhzVgg5QEkMvOFCuqFX49h0jWpH20pne3FZvT71Lcd-3UPKesZ03MB4CPukmZRCKNr28t-okB1vOtqzgvYntNiXUoRR7yLOJt5pRvXRYb3Vfzisjw5rynVxuGifn8fshxncL-VPSwvw6gyYZM00RuMtpt9cJ9pedLRwL07caII2n2NhbtdlkqRlgYbT40WrEwHlwweEqJNF8BYcRrBZu4D_sfA9p0LYXg</recordid><startdate>20130515</startdate><enddate>20130515</enddate><creator>Caballero Gómez, Natacha</creator><creator>Abriouel, Hikmate</creator><creator>Grande, Mª. José</creator><creator>Pérez Pulido, Rubén</creator><creator>Gálvez, Antonio</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130515</creationdate><title>Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells</title><author>Caballero Gómez, Natacha ; Abriouel, Hikmate ; Grande, Mª. José ; Pérez Pulido, Rubén ; Gálvez, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-8783b1e8724ec6eed2e0532a593a1ddc334be532b25234db98ff57586fc64dc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteriocin</topic><topic>bacteriocins</topic><topic>Bacteriocins - pharmacology</topic><topic>benzalkonium chloride</topic><topic>Biocides</topic><topic>Biofilm</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>bromides</topic><topic>cell growth</topic><topic>chlorhexidine</topic><topic>chlorides</topic><topic>cleaning</topic><topic>Disinfectants - pharmacology</topic><topic>disinfection</topic><topic>Disinfection - standards</topic><topic>Food industries</topic><topic>food industry</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hexachlorophene</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - physiology</topic><topic>Microbial Viability - drug effects</topic><topic>Plankton - drug effects</topic><topic>polystyrenes</topic><topic>Staphylococcus</topic><topic>Staphylococcus aureus</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caballero Gómez, Natacha</creatorcontrib><creatorcontrib>Abriouel, Hikmate</creatorcontrib><creatorcontrib>Grande, Mª. José</creatorcontrib><creatorcontrib>Pérez Pulido, Rubén</creatorcontrib><creatorcontrib>Gálvez, Antonio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caballero Gómez, Natacha</au><au>Abriouel, Hikmate</au><au>Grande, Mª. José</au><au>Pérez Pulido, Rubén</au><au>Gálvez, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2013-05-15</date><risdate>2013</risdate><volume>163</volume><issue>2-3</issue><spage>96</spage><epage>100</epage><pages>96-100</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Control of staphylococci during cleaning and disinfection is important to the food industry. Broad-spectrum bacteriocins with proved anti-staphylococcal activity, such as enterocin AS-48, could open new possibilities for disinfection in combination with biocides. In the present study, enterocin AS-48 was tested singly or in combination with biocides against a cocktail of six Staphylococcus aureus strains (including three methicillin-resistant strains) in planktonic state as well as in biofilms formed on polystyrene microtiter plates. Cells were challenged with enterocin, biocides or enterocin/biocide combinations. Inactivation of planktonic cells increased significantly (p<0.05) when enterocin AS-48 (25mg/l) was tested in combination with benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and non-significantly in combination with didecyldimethylammonium bromide (AB), triclosan (TC), hexachlorophene (CF), polyhexamethylen guanidinium chloride (PHMG), chlorhexidine (CH) or P3-oxonia (OX). In the sessile state (24h biofilms), staphylococci required higher biocide concentrations in most cases, except for OX. Inactivation of sessile staphylococci increased remarkably when biocides were applied in combination with enterocin AS-48, especially when the bacteriocin was added at 50mg/l. During storage, the concentrations of sessile as well as planktonic cells in the treated samples decreased remarkably for BC, TC and PHMG, but OX failed to inhibit proliferation of the treated biofilms as well as growth of planktonic cells. The observed inhibitory effects during storage were potentiated when the biocides were combined with 50mg/l enterocin AS-48. Results from this study suggest that selected combinations of enterocin AS-48 and biocides offer potential use against planktonic and sessile, methicillin-sensitive and methicillin-resistant S. aureus.
•A cocktail of methicillin resistant and sensitive staphylococci was sensitive to biocides and enterocin AS-48.•Combined treatments of enterocin/biocide improved inactivation of planktonic and sessile staphylococci.•The combined treatments delayed or avoided recovery of treated biofilms during storage.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23558192</pmid><doi>10.1016/j.ijfoodmicro.2013.02.018</doi><tpages>5</tpages></addata></record> |
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| ispartof | International journal of food microbiology, 2013-05, Vol.163 (2-3), p.96-100 |
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| subjects | Bacteriocin bacteriocins Bacteriocins - pharmacology benzalkonium chloride Biocides Biofilm Biofilms - drug effects Biofilms - growth & development Biological and medical sciences bromides cell growth chlorhexidine chlorides cleaning Disinfectants - pharmacology disinfection Disinfection - standards Food industries food industry Food microbiology Fundamental and applied biological sciences. Psychology hexachlorophene Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - physiology Microbial Viability - drug effects Plankton - drug effects polystyrenes Staphylococcus Staphylococcus aureus Time Factors |
| title | Combined treatments of enterocin AS-48 with biocides to improve the inactivation of methicillin-sensitive and methicillin-resistant Staphylococcus aureus planktonic and sessile cells |
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