Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures

• Published in: Plant physiology and biochemistry 2013-10, Vol.71, p.144-154
• Main Authors: Carvalho, Camila Pereira, Hayashi, Adriana Hissae, Braga, Marcia Regina, Nievola, Catarina Carvalho
• Format: Article
• Language: English
• Subjects: acclimation; Biological and medical sciences; bleaching; Bromeliaceae; Bromeliaceae - anatomy & histology; Bromeliaceae - metabolism; Bromeliaceae - physiology; Carbohydrates; carbon; cell walls; chlorophyll; Cold Temperature; conservation programs; Ex vitro acclimatization; Fundamental and applied biological sciences. Psychology; in vitro culture; Leaf plasticity; leaves; Low temperature functional adaptation; parenchyma; Pectin; pectins; plant growth; Plant Leaves - anatomy & histology; Plant Leaves - metabolism; Plant Leaves - physiology; Plant physiology and development; reducing sugars; survival rate; temperature
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2013.07.005

Nidularium minutum is a tropical bromeliad that grows in natural environment with temperatures ranging from 2 to 30 °C. In the present work we cultivated this species in vitro at 5, 10, 15, and 25 °C for 3 and 6 months aiming at assessing biochemical and morphological responses that allow its survival under low temperatures. No survival was observed for plants cultured constantly at 5 °C and the lowest biometric parameters were found for those grown at 10 °C. A thick aquiferous parenchyma, accumulation of reducing sugars, and increased pectin content in the cell walls were observed in plants grown at 10 and 15 °C when compared to those maintained at 25 °C. In plants cultured at 10 °C, leaf bleaching correlated with low chlorophyll content and lower survival rate after 6 months when compared to those grown at 15 °C. The best in vitro culture condition for slow growth and plant acclimatization was found to be at 15 °C. This probably correlated with the immediate availability of carbon to restore growth during acclimatization and also with higher root initiation under this condition. This study brings information about the responses related to functional adaptation to low temperatures in N. minutum cultured in vitro that can also be implicated in its survival under natural conditions. Additionally, it suggests the best temperature to form a minimal growth collection to be used in restocking and conservation programs for endangered tropical bromeliads. •The bromeliad Nidularium minutum cultivated in vitro survived at low temperatures.•The best in vitro culture condition for slow growth was 15 °C.•The tolerance to low temperatures was consistent with increased reducing sugars.•Maintenance of the water status correlated with a thicker aquiferous parenchyma.•Survival of acclimatized plants was related to starch content and root initiation.