Tubule detection in testis images using boundary weighting and circular shortest path

In studies of germ cell transplantation, measureing tubule diameters and counting cells from different populations using antibodies as markers are very important. Manual measurement of tubule sizes and cell counts is a tedious and sanity grinding work. In this paper, we propose a new boundary weight...

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Bibliographic Details
Main Authors: Chao Zhang, Changming Sun, Davey, Rhonda, Ran Su, Bischof, Leanne, Vallotton, Pascal, Lovell, David, Hope, Shelly, Lehnert, Sigrid, Pham, Tuan D.
Format: Conference Proceeding
Language:English
Subjects:
Australia
Biology
Image edge detection
Image segmentation
Informatics
Manuals
Transforms
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Summary:In studies of germ cell transplantation, measureing tubule diameters and counting cells from different populations using antibodies as markers are very important. Manual measurement of tubule sizes and cell counts is a tedious and sanity grinding work. In this paper, we propose a new boundary weighting based tubule detection method. We first enhance the linear features of the input image and detect the approximate centers of tubules. Next, a boundary weighting transform is applied to the polar transformed image of each tubule region and a circular shortest path is used for the boundary detection. Then, ellipse fitting is carried out for tubule selection and measurement. The algorithm has been tested on a dataset consisting of 20 images, each having about 20 tubules. Experiments show that the detection results of our algorithm are very close to the results obtained manually.
ISSN:1094-687X
1558-4615
2694-0604
DOI:10.1109/EMBC.2013.6610251