Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR(-/-) mice by FT-IR imaging

In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1...

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Bibliographic Details
Published in:Analyst (London) 2013-11, Vol.138 (21), p.6645-6652
Main Authors: Wrobel, Tomasz P, Mateuszuk, Lukasz, Kostogrys, Renata B, Chlopicki, Stefan, Baranska, Malgorzata
Format: Article
Language:English
Subjects:
Animals
Apolipoproteins E - deficiency
Atherosclerosis - metabolism
Disease Models, Animal
Disease Progression
Female
Mice
Mice, Inbred C57BL
Mice, Knockout
Plaque, Atherosclerotic - chemistry
Plaque, Atherosclerotic - metabolism
Receptors, LDL - deficiency
Spectroscopy, Fourier Transform Infrared - methods
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Summary:In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an01050c