Low concentration of PDGF-AB shows synergism with IFN-α in induction of IFN-β and -γ in MRC5 fibroblasts

•We show that PDGF-AB and IFN-α, produce significant induction of IFN-α, -β and -γ.•The induction was dose-dependent.•It was the highest when IFN-α was combined with the lowest concentration of PDGF-AB.•Results presented here open new possibilities in multi-cytokine therapy. Platelet-derived growth...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2013-11, Vol.64 (2), p.494-496
Main Authors: Šantak, G., Šantak, M., Forčić, D.
Format: Article
Language:English
Subjects:
Cell Line
chemotaxis
cytokines
fibroblasts
Fibroblasts - drug effects
Fibroblasts - metabolism
Gene Expression Regulation - drug effects
Humans
Interferon-alpha - genetics
Interferon-alpha - metabolism
Interferon-alpha - pharmacology
Interferon-beta - genetics
Interferon-beta - metabolism
Interferon-gamma - genetics
Interferon-gamma - metabolism
Interferon-α
Interferon-β
Interferon-γ
interferons
PDGF-AB
platelet-derived growth factor
Platelet-Derived Growth Factor - pharmacology
RNA, Messenger - genetics
RNA, Messenger - metabolism
synergism
Synergy
therapeutics
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Summary:•We show that PDGF-AB and IFN-α, produce significant induction of IFN-α, -β and -γ.•The induction was dose-dependent.•It was the highest when IFN-α was combined with the lowest concentration of PDGF-AB.•Results presented here open new possibilities in multi-cytokine therapy. Platelet-derived growth factor (PDGF) is a potent mediator of fibroblast proliferation and chemotaxis. Also it has been reported as a strong suppressor of interferon (IFN) expression. IFN-α has opposite effect on fibroblast function and IFN induction. Here is our early report on the effect of low concentration of PDGF-AB alone or in combination with IFN-α on IFN mRNA production in MRC5 fibroblasts. MRC5 cells incubated with IFN-α or PDGF-AB, alone or in combination, produced significant induction of IFN-α, -β and -γ mRNA in comparison with untreated cells. The induction was dose-dependent, with higher effect in cells treated with lower concentrations of PDGF-AB. Also, low concentration of PDGF-AB showed synergism with IFN-α in IFN-β and -γ induction. Results presented here open new possibilities in multi-cytokine therapy and expand previous results on PDGF activity.
ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2013.09.001