Low concentration of PDGF-AB shows synergism with IFN-α in induction of IFN-β and -γ in MRC5 fibroblasts

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2013-11, Vol.64 (2), p.494-496
• Main Authors: Šantak, G., Šantak, M., Forčić, D.
• Format: Article
• Language: English
• Subjects: Cell Line; chemotaxis; cytokines; fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene Expression Regulation - drug effects; Humans; Interferon-alpha - genetics; Interferon-alpha - metabolism; Interferon-alpha - pharmacology; Interferon-beta - genetics; Interferon-beta - metabolism; Interferon-gamma - genetics; Interferon-gamma - metabolism; Interferon-α; Interferon-β; Interferon-γ; interferons; PDGF-AB; platelet-derived growth factor; Platelet-Derived Growth Factor - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; synergism; Synergy; therapeutics
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2013.09.001

•We show that PDGF-AB and IFN-α, produce significant induction of IFN-α, -β and -γ.•The induction was dose-dependent.•It was the highest when IFN-α was combined with the lowest concentration of PDGF-AB.•Results presented here open new possibilities in multi-cytokine therapy. Platelet-derived growth factor (PDGF) is a potent mediator of fibroblast proliferation and chemotaxis. Also it has been reported as a strong suppressor of interferon (IFN) expression. IFN-α has opposite effect on fibroblast function and IFN induction. Here is our early report on the effect of low concentration of PDGF-AB alone or in combination with IFN-α on IFN mRNA production in MRC5 fibroblasts. MRC5 cells incubated with IFN-α or PDGF-AB, alone or in combination, produced significant induction of IFN-α, -β and -γ mRNA in comparison with untreated cells. The induction was dose-dependent, with higher effect in cells treated with lower concentrations of PDGF-AB. Also, low concentration of PDGF-AB showed synergism with IFN-α in IFN-β and -γ induction. Results presented here open new possibilities in multi-cytokine therapy and expand previous results on PDGF activity.