Determination of cefdinir levels in rat plasma and urine by high-performance liquid chromatography-tandem mass spectrometry: application to pharmacokinetics after oral and intravenous administration of cefdinir

• Published in: Biomedical chromatography 2013-11, Vol.27 (11), p.1423-1430
• Main Authors: Jin, Hyo-Eon, Kim, In-Bong, Kim, Chong-Kook, Maeng, Han-Joo
• Format: Article
• Language: English
• Subjects: Administration, Intravenous; Administration, Oral; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - blood; Anti-Bacterial Agents - urine; cefdinir; Cephalosporins - administration & dosage; Cephalosporins - blood; Cephalosporins - urine; Chromatography, High Pressure Liquid - methods; LC-MS/MS; Limit of Detection; Male; pharmacokinetics; plasma; rat; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry - methods; urine
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.2938

ABSTRACT A selective and sensitive liquid chromatography tandem mass spectrometry method (LC‐MS/MS) was developed and validated for the determination of cefdinir in rat plasma and urine. Following a simple protein precipitation using methanol, chromatographic separation was achieved with a run time of 10 min using a Synergi 4 µ polar‐RP 80A column (150 × 2.0 mm, 4 µm) with a mobile phase consisting of 0.1% formic acid in water and methanol (65:35, v/v) at a flow rate of 0.2 mL/min. The protonated precursor and product ion transitions for cefdinir (m/z 396.1 → 227.2) and cefadroxil, an internal standard (m/z 364.2 → 208.0) were monitored in the multiple reaction monitoring in positive ion mode. The calibration curves for plasma and urine were linear over the concentration range 10–10,000 ng/mL. The lower limit of quantification was 10 ng/mL. All accuracy values were between 95.1 and 113.0% and the intra‐ and inter‐day precisions were