Allicin Induces Anti-human Liver Cancer Cells through the p53 Gene Modulating Apoptosis and Autophagy

Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer globally and ranks first among the cancer-related mortalities in Taiwan. This study aims to understand the modes of cell death mechanism induced by allicin, a major phytochemical of crushed garlic, in human hepatoma cells. Our...

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Published in:Journal of agricultural and food chemistry 2013-10, Vol.61 (41), p.9839-9848
Main Authors: Chu, Yung-Lin, Ho, Chi-Tang, Chung, Jing-Gung, Raghu, Rajasekaran, Lo, Yi-Chen, Sheen, Lee-Yan
Format: Article
Language:English
Subjects:
allicin
apoptosis
Apoptosis - drug effects
autophagy
Autophagy - drug effects
Biological and medical sciences
complementary genes
Fundamental and applied biological sciences. Psychology
Garlic - chemistry
Hep G2 Cells
hepatoma
Humans
Liver Neoplasms - drug therapy
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - physiopathology
Plant Extracts - pharmacology
reactive oxygen species
Sulfinic Acids - pharmacology
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
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Summary:Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer globally and ranks first among the cancer-related mortalities in Taiwan. This study aims to understand the modes of cell death mechanism induced by allicin, a major phytochemical of crushed garlic, in human hepatoma cells. Our earlier study indicated that allicin induced autophagic cell death in human HCC Hep G2 (p53 wild type) cells, whereas in the present study, allicin induced apoptotic cell death through caspase-dependent and caspase-independent pathways by reactive oxygen species (ROS) overproduction in human HCC Hep 3B (p53 mutation) cells. To gain insight into the cell death mechanism in p53 knocked down Hep G2, we silenced the p53 gene using siRNA-mediated silencing. Allicin treatment induced apoptotic cell death in p53 knocked down Hep G2 cells similar to that of Hep 3B cells. These results suggest that allicin induced cell death in human hepatoma cells through either autophagy or apoptosis and might be a potential novel complementary gene therapeutic agent for the treatment of apoptosis-resistant cancer cells.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf403241s