Histopathological Studies on Cases of Chronic Mouse Hepatitis by Natural Helicobacter Infections

It is known that Helicobacter hepaticus or Helicobacter bilis infection causes chronic inflammation of the colon and liver. Chronic active hepatitis was found in radiation exposure experiments using male C3H/HeNrs mice at our institute. Histopathologically, 103 cases among 978 mice (64–91 weeks of a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Veterinary Medical Science 2013, Vol.75(9), pp.1231-1235
Main Authors: NAM, Chunja, OHMACHI, Yasushi, KOKUBO, Toshiaki, NISHIKAWA, Tetsu, UCHIDA, Kazuyuki, NAKAYAMA, Hiroyuki
Format: Article
Language:English
Subjects:
Animals
Animals, Laboratory - microbiology
C3H/HeNrs mouse
chronic hepatitis
Helicobacter
Helicobacter Infections - epidemiology
Helicobacter Infections - pathology
Helicobacter Infections - veterinary
Hepatitis, Animal - epidemiology
Hepatitis, Animal - pathology
Incidence
Liver - pathology
Male
Mice
Mice, Inbred Strains
natural infection
Rodent Diseases - epidemiology
Rodent Diseases - microbiology
Rodent Diseases - pathology
Whole-Body Irradiation - adverse effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is known that Helicobacter hepaticus or Helicobacter bilis infection causes chronic inflammation of the colon and liver. Chronic active hepatitis was found in radiation exposure experiments using male C3H/HeNrs mice at our institute. Histopathologically, 103 cases among 978 mice (64–91 weeks of age at autopsy) had hepatic lesions regardless of irradiation exposure. Mild lesions showed only focal necrosis and focal inflammation in the liver. Severe cases were accompanied by hepatocytomegaly, bile duct hyperplasia, hypertrophy and activation of Kupffer cells, cholangitis, pleomorphic hepatocytes and/or tumor. Helical-shaped bacteria were detected between hepatocytes by Warthin-Starry silver stain and immunohistochemistry (IHC) with an antibody against Helicobacter pylori. It was suggested that these cases of chronic hepatitis were caused by Helicobacter spp. Although chronic hepatitis occurred frequently in mice exposed high-dose irradiation compared with nonirradiated mice in one lot, it was not concluded that radiation might influence the incidence or degree of hepatitis. Our report suggested that natural Helicobacter spp. infection in mice can occur in an experimental animal facility. Therefore, it is suggested that monitoring of Helicobacter infection is very important for quality control of animal experiments.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.12-0304