Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed- and chiral-phase LC-MS

• Published in: Journal of separation science 2013-10, Vol.36 (20), p.3310-3320
• Main Authors: Řezanka, Tomáš, Kolouchová, Irena, Čejková, Alena, Cajthaml, Tomáš, Sigler, Karel
• Format: Article
• Language: English
• Subjects: Atmospheric pressure; Atmospheric pressure chemical ionization mass spectrometry; Biological and medical sciences; Chiral LC; Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase - methods; Fundamental and applied biological sciences. Psychology; Ions; Mass spectrometry; Microbiology; Morphology, structure, chemical composition; Mycology; Reversed phase LC; Stereoisomerism; Triacylglycerols; Triglycerides - chemistry; Triglycerides - metabolism; Yeast; Yeasts - chemistry; Yeasts - classification; Yeasts - metabolism
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201300657

LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols (TAGs) from yeasts of the genera Candida, Kluyveromyces, Rhodotorula, Saccharomyces, Torulospora, Trichosporon, and Yarrowia. Chiral LC–APCI‐MS is based on using two columns in series packed with a 3,5‐dimethylphenyl carbamate modified β‐cyclodextrin chiral phase. All regioisomers and enantiomers of TAGs containing one to five double bonds were separated. Molecular species of TAGs, i.e. regioisomers and enantiomers, were identified and quantified by MS/MS. Among the 94 identified TAGs, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids (FAs), this acid, or unsaturated FA is bound in sn‐1. In strains containing palmitoleic acid at 10–25% total FAs this acid is mainly bound in sn‐3, saturated FA being bound in sn‐1. Strains containing