Nothing is perfect! Trouble-shooting in immunological and molecular studies of cestode infections

This personal review focuses on ways to approach and overcome some of the more common issues encountered while studying cestode zoonoses. The information presented here is based on the author's own experiences with immunological and molecular approaches for the detection of these parasites. The...

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Bibliographic Details
Published in:Parasitology 2013-11, Vol.140 (13), p.1551-1565
Main Author: ITO, AKIRA
Format: Article
Language:English
Subjects:
Animals
Anthelmintics - therapeutic use
Antibodies, Helminth - analysis
Antigens, Helminth - analysis
Biomarkers - analysis
Cestoda - isolation & purification
Cestoda - physiology
Cysticercosis - diagnosis
Cysticercosis - drug therapy
Cysticercosis - immunology
Developing Countries
Echinococcosis - diagnosis
Echinococcosis - drug therapy
Echinococcosis - immunology
Humans
Immune Sera - analysis
Immunoassay - standards
LDCs
Parasites
Reagent Kits, Diagnostic
Zoonoses - diagnosis
Zoonoses - immunology
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Description
Summary:This personal review focuses on ways to approach and overcome some of the more common issues encountered while studying cestode zoonoses. The information presented here is based on the author's own experiences with immunological and molecular approaches for the detection of these parasites. There are many incongruities between immunological and molecular studies due to biased work. Nothing is perfect. Indirect approaches using either immunological, or even molecular tools, are limited without confirmation from direct evidence of infection. The dilemma of whether developing countries should develop their own diagnostic tests or rely on commercially available kits is also discussed.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182013000966