Immunohistochemical analysis of the DNA methyltransferase 3b expression is associated with significant improvements in the discrimination of ulcerative colitis-associated neoplastic lesions

Purpose Making a clinicopathological diagnosis of dysplasia is crucial. We herein assess the significance of the DNA methyltransferase 3b (DNMT3b) expression as a diagnostic marker of ulcerative colitis (UC)-associated neoplasia. Methods Thirty-one patients with long-standing and extensive UC were i...

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Published in:Surgery today (Tokyo, Japan) Japan), 2013-11, Vol.43 (11), p.1275-1280
Main Authors: Ueda, Hirofumi, Tanaka, Hiroyuki, Ichikawa, Kazuhito, Itabashi, Michio, Kameoka, Shingo, Fujii, Shigehiko, Saito, Natsuko, Kimura, Ryusuke, Shida, Yosuke, Fujimori, Yukari, Ohtake, Shinichirou, Tomita, Shigeki, Imura, Johji, Yasuda, Yoshikazu, Tanigawa, Nobuhiko, Uchiyama, Kazuhisa, Fujimori, Takahiro
Format: Article
Language:English
Subjects:
Adult
Biomarkers - analysis
Colitis, Ulcerative - complications
Colitis, Ulcerative - diagnosis
Colorectal Neoplasms - diagnosis
Colorectal Neoplasms - etiology
Diagnosis, Differential
DNA (Cytosine-5-)-Methyltransferases - analysis
DNA Methyltransferase 3B
Female
Humans
Immunohistochemistry - methods
Male
Medicine
Medicine & Public Health
Middle Aged
Original Article
Surgery
Surgical Oncology
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Summary:Purpose Making a clinicopathological diagnosis of dysplasia is crucial. We herein assess the significance of the DNA methyltransferase 3b (DNMT3b) expression as a diagnostic marker of ulcerative colitis (UC)-associated neoplasia. Methods Thirty-one patients with long-standing and extensive UC were included in this study. The expression of DNMT3b in non-neoplastic rectal epithelium (non-dysplasia in 31 patients) and colorectal neoplasia (dysplasia in 43 patients and invasive cancer in 34 patients) was determined using immunohistochemistry. The presence of immunoreactive DNMT3b was assessed in the areas with the highest density of cells with positively staining nuclei. DNMT3b was expressed as the percentage of positive cells relative to the total number of cells counted under high power magnification. Results The DNMT3b expression in neoplastic rectal epithelium (0.76, range 0.59–0.84) was increased compared to that observed in non-neoplastic epithelium (0.32, range 0.18–0.67, P  
ISSN:0941-1291
1436-2813
DOI:10.1007/s00595-012-0456-6