Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris

The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were...

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Bibliographic Details
Published in:Biotechnology letters 2013-11, Vol.35 (11), p.1865-1871
Main Authors: Liang, Shuli, Zou, Chengjuan, Lin, Ying, Zhang, Xuanwei, Ye, Yanrui
Format: Article
Language:English
Subjects:
Gene Expression
Genes, Reporter
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Pichia - genetics
Promoter Regions, Genetic
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
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Summary:The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity was analyzed by measuring fluorescence intensity. P GCW14 exhibited significantly stronger promoter activity than the classic strong constitutive promoters P TEF1 and P GAP under various carbon sources, suggesting that P GCW14 is a strong and constitutive promoter. Hence, P GCW14 can be used as a promoter for high-level expression of heterologous proteins.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1265-8