Design of polyethylene glycol-polyethylenimine nanocomplexes as non-viral carriers: mir-150 delivery to chronic myeloid leukemia cells

MicroRNAs (miRNAs) are acknowledged as indispensable regulators relevant in many biological processes, and they have been pioneered as therapeutic targets for curing disease. miRNAs are single‐stranded, small (19–22 nt) regulatory non‐coding RNAs whose deregulation of expression triggers human cance...

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Published in:Cell biology international 2013-11, Vol.37 (11), p.1205-1214
Main Authors: Biray Avcı, Çığır, Özcan, İpek, Balcı, Tuğçe, Özer, Özgen, Gündüz, Cumhur
Format: Article
Language:English
Subjects:
Cell Death
Cell Line, Tumor
Electrophoretic Mobility Shift Assay
Flow Cytometry
Gene Transfer Techniques
Genetic Vectors - metabolism
Humans
leukemia
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism
microRNA
MicroRNAs - metabolism
Microscopy, Fluorescence
nanoparticles
Nanoparticles - chemistry
Particle Size
poly(ethylene glycol)
Polyethylene Glycols - chemistry
Polyethyleneimine - analogs & derivatives
Polyethyleneimine - chemistry
polyethylenimine
Reproducibility of Results
Static Electricity
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Summary:MicroRNAs (miRNAs) are acknowledged as indispensable regulators relevant in many biological processes, and they have been pioneered as therapeutic targets for curing disease. miRNAs are single‐stranded, small (19–22 nt) regulatory non‐coding RNAs whose deregulation of expression triggers human cancers, including leukemias, mainly through dysregulation of expression of leukemia genes. miRNAs can function as tumour suppressors (suppressing malignant potential) or oncogenes (activating malignant potential) like actors of complex diseases. To address the issue of overcoming instability and low transfection efficiency in vitro, the polyethylene glycol–polyethyleneimine (PEG–PEI) nanoparticle was used as non‐viral vector carrier for miR‐150 transfection, which is downregulated in chronic myeloid leukemia. PEG–PEI [PEG(550)3‐g‐PEI(1800)]/miRNA nanocomplexes were synthesised and characterised by particle size distribution (PSD), polydispersity index (PDI) and zeta potential, surface charge, their cytotoxicity, and transfection efficiency. Interaction with human leukemia cells (K‐562 and KU812) and control cells NCI‐BL2347 with them has been investigated. The transfection efficiency of PEG–PEI/miRNA at N/P 26 rose 6.7‐fold above the control by qRT‐PCR. The size of homogenous nanocomplexes (PBI 
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10157