Comparative Characteristics of Lipemia Models Induced by Injections of Triton WR-1339 and Poloxamer 407 in Mice

• Published in: Bulletin of experimental biology and medicine 2013-06, Vol.155 (2), p.284-287
• Main Authors: Loginova, V. M., Tuzikov, F. V., Tuzikova, N. A., Korolenko, T. A.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cholesterol; Cholesterol - blood; Disease Models, Animal; Hyperlipidemias - chemically induced; Hyperlipidemias - physiopathology; Internal Medicine; Laboratory Medicine; Lipoproteins, HDL - blood; Lipoproteins, HDL - drug effects; Lipoproteins, LDL - blood; Lipoproteins, LDL - drug effects; Low density lipoproteins; Macrophages - drug effects; Methods; Mice; Pathology; Poloxamer - pharmacology; Polyethylene Glycols - pharmacology; Surface-Active Agents - pharmacology; Triglycerides - blood
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-2133-1

Lipemia modeled by injections of Triton WR-1339 (500 mg/kg) and poloxamer 407 (500 mg/kg) to mice were compared. LP fraction and subfraction compositions were compared by small-angle X-ray scattering on a diffractometer. Both compounds in the same dose caused a sharp increase in serum concentrations of total cholesterol (CH) and triglycerides (TG), the increases in response to poloxamer 407 being more pronounced. The differences in the models consisted in the levels of atherogenic fractions: CH-VLDL (subfractions CH-VLDL 1-2 ) and CH-LDL, which were higher under the effect of poloxamer 407. Similar increases were observed for atherogenic fractions: TG-VLDL (subfractions TG-VLDL 1-2 ) and TG-LDL (subfractions TG-LDL 1-3 ). A specifi c feature of the model induced by poloxamer 407 was elevation of the concentrations of antiatherogenic CH-HDL and TG-HDL (subfractions CH-HDL 2 and TG-HDL 2 ). Both models exhibited high similarity, but changes in atherogenic fractions were more pronounced under the effect of poloxamer 407.