Osteoblast responses to thin nanohydroxyapatite coated on roughened titanium surfaces deposited by an electrochemical process

Purpose The purpose of this study was to investigate in vitro osteoblast responses to the thin nano-hydroxyapatite (HA) coating on porous implant surfaces. Materials and Methods Surface characteristics of nano-HA coating were evaluated by x-ray diffractometer (XRD) and Fourier transform infrared spe...

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Published in:Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2013-11, Vol.116 (5), p.e311-e316
Main Authors: Shi, Jue, MA, DDS, Dong, Ling Ling, BA, DDS, He, Fuming, PhD, DDS, Zhao, Shifang, MD, DDS, Yang, Guo-Li, PhD, DDS
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Cell Proliferation
Cells, Cultured
Dental Implants
Dentistry
Durapatite - chemistry
Electrochemical Techniques
Materials Testing
Mice
Nanotechnology
Osteoblasts - cytology
Osteoblasts - metabolism
Osteocalcin - metabolism
Porosity
Spectroscopy, Fourier Transform Infrared
Surface Properties
Surgery
Titanium - chemistry
X-Ray Diffraction
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Summary:Purpose The purpose of this study was to investigate in vitro osteoblast responses to the thin nano-hydroxyapatite (HA) coating on porous implant surfaces. Materials and Methods Surface characteristics of nano-HA coating were evaluated by x-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). Murine preosteoblast cell (MC3T3-E1) proliferation, alkaline phosphatase (ALP) activity, and osteocalcin release on nano-HA coated surfaces were compared with HA-coated surfaces. Results The XRD pattern demonstrated that the peak of nano-HA coating matched well with the standard HA patterns. FTIR spectra also showed that the coating consisted of pure HA crystals. Significant increases in cell proliferation, total protein on day 7, ALP activity on day 14 and day 21, and osteocalcin production on day 21 ( P < .05) were observed for nano-HA coated surfaces. Conclusions It was concluded that thin nano-HA coating, deposited by the electrochemical process, improved proliferation and differentiation of osteoblasts.
ISSN:2212-4403
2212-4411
DOI:10.1016/j.oooo.2012.02.021