The regulation of growth and metabolism of kidney stem cells with regional specificity using extracellular matrix derived from kidney

Abstract Native extracellular matrix (ECM) that is secreted and maintained by resident cells is of great interest for cell culture and cell delivery. We hypothesized that specialized bioengineered niches for stem cells can be established using ECM-derived scaffolding materials. Kidney was selected a...

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Bibliographic Details
Published in:Biomaterials 2013-12, Vol.34 (38), p.9830-9841
Main Authors: O'Neill, John D, Freytes, Donald O, Anandappa, Annabelle J, Oliver, Juan A, Vunjak-Novakovic, Gordana V
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cells, Cultured
Dentistry
Extracellular matrix
Extracellular Matrix - metabolism
Extracellular Matrix - ultrastructure
Hydrogels
Immunohistochemistry
Kidney
Kidney - cytology
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - ultrastructure
Mice
Microscopy, Electron, Scanning
Niche
Regional specificity
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Stem Cells - ultrastructure
Swine
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Summary:Abstract Native extracellular matrix (ECM) that is secreted and maintained by resident cells is of great interest for cell culture and cell delivery. We hypothesized that specialized bioengineered niches for stem cells can be established using ECM-derived scaffolding materials. Kidney was selected as a model system because of the high regional diversification of renal tissue matrix. By preparing the ECM from three specialized regions of the kidney (cortex, medulla, and papilla; whole kidney, heart, and bladder as controls) in three forms: ( i ) intact sheets of decellularized ECM, ( ii ) ECM hydrogels, and ( iii ) solubilized ECM, we investigated how the structure and composition of ECM affect the function of kidney stem cells (with mesenchymal stem cells, MSCs, as controls). All three forms of the ECM regulated KSC function, with differential structural and compositional effects. KSCs cultured on papilla ECM consistently displayed lower proliferation, higher metabolic activity, and differences in cell morphology, alignment, and structure formation as compared to KSCs on cortex and medulla ECM, effects not observed in corresponding MSC cultures. These data suggest that tissue- and region-specific ECM can provide an effective substrate for in vitro studies of therapeutic stem cells.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.09.022