Characterization of prostaglandin E2 receptors (EP2, EP4) in the horse oviduct

Biological effects of prostaglandin E₂ are mediated via one of four receptors designated EP1, EP2, EP3 and EP4 which are encoded by separate genes. In general, EP1 and EP3 induce smooth muscle contraction whereas EP2 and EP4 induce smooth muscle relaxation. The objective of the current study was to...

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Bibliographic Details
Published in:Animal reproduction science 2013-11, Vol.142 (1-2), p.35-41
Main Authors: Ball, B.A, Scoggin, K.E, Troedsson, M.H.T, Squires, E.L
Format: Article
Language:English
Subjects:
Animals
EP2
EP4
epithelium
estrous cycle
Estrous Cycle - physiology
Fallopian Tubes - physiology
Female
Gene Expression Regulation - physiology
genes
Horse
Horses - physiology
mares
messenger RNA
muscle contraction
Oviduct
Prostaglandin E2
Prostaglandin E2 receptor
prostaglandins
quantitative polymerase chain reaction
receptors
Receptors, Prostaglandin E, EP2 Subtype - genetics
Receptors, Prostaglandin E, EP2 Subtype - metabolism
Receptors, Prostaglandin E, EP4 Subtype - genetics
Receptors, Prostaglandin E, EP4 Subtype - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
smooth muscle
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Summary:Biological effects of prostaglandin E₂ are mediated via one of four receptors designated EP1, EP2, EP3 and EP4 which are encoded by separate genes. In general, EP1 and EP3 induce smooth muscle contraction whereas EP2 and EP4 induce smooth muscle relaxation. The objective of the current study was to characterize the expression of the genes for PGE₂ receptors (EP2 and EP4) in the horse oviduct based upon immunohistochemistry (IHC) and quantitative PCR (qPCR). Oviductal tissue was collected from mares at estrus (n=5), at 5 days post-ovulation (n=4), and from prepubertal mares (n=5). Isthmic and ampullar regions of the oviduct were fixed for IHC or preserved for RNA isolation. Prostaglandin E₂ receptors EP2 and EP4 were strongly expressed by the luminal epithelium of both the isthmic and ampullar regions of the horse oviduct with a lesser immuno-expression noted within the smooth muscle in both regions of the oviduct. Based upon qPCR, relative amounts of EP2 or EP4 mRNA did not differ across estrous cycle stage or from prepubertal mares. However, across region and estrous cycle stage, relative amount of EP2 was greater (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2013.07.009