Development of an Enzyme-Linked Immunosorbent Assay for 3,5,6-Trichloro-2-pyridinol. 1. Production and Characterization of Monoclonal Antibodies

3,5,6-Trichloro-2-pyridinol (TCP) is the major degradation product of both the widely used chlorpyrifos and chlorpyrifos-methyl insecticides and the herbicide triclopyr. In this paper we describe the development of immunoassays for the detection of this metabolite. With this aim, two types of hapten...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1996-11, Vol.44 (11), p.3703-3709
Main Authors: Manclús, Juan J, Montoya, Angel
Format: Article
Language:English
Subjects:
Agrochemicals products
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Biological and medical sciences
CHLORPYRIFOS
CHLORPYRIFOS METHYL
CLORPIRIFOS
CLORPIRIFOS METIL
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DEGRADACION
DEGRADATION
ELISA
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
METABOLITE
METABOLITOS
Methods of analysis, processing and quality control, regulation, standards
PESTICIDE
Pesticides used against invertebrates
Phytopathology. Animal pests. Plant and forest protection
PLAGUICIDAS
Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors
Pollution effects. Side effects of agrochemicals
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
TEST ELISA
TRICLOPIR
TRICLOPYR
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Summary:3,5,6-Trichloro-2-pyridinol (TCP) is the major degradation product of both the widely used chlorpyrifos and chlorpyrifos-methyl insecticides and the herbicide triclopyr. In this paper we describe the development of immunoassays for the detection of this metabolite. With this aim, two types of haptens were synthesized, with spacer attachment either through the 6-position of the pyridyl ring or through the hydroxyl group. Protein conjugates of these haptens were used for the immunization of mice to produce monoclonal antibodies (MAbs). From haptens of the former type, a panel of MAbs displaying 50% inhibition (I 50) for TCP in the range 1−10 nM was obtained. Characterization of these MAbs showed their high affinity and specificity for TCP. The best MAbs were used in various assay formats in combination with homologous and heterologous assay haptens. Several immunoassays with I 50 lower than 1 nM were developed. These immunoassays are potentially very valuable analytical tools for the determination of TCP in food and in the environment, as well as for monitoring human exposure to chlorpyrifos. Keywords: 3,5,6-Trichloro-2-pyridinol (TCP); chlorpyrifos; monoclonal antibodies; ELISA
ISSN:0021-8561
1520-5118
DOI:10.1021/jf950656p