Anticonvulsant and sedative effects of paederosidic acid isolated from Paederia scandens (Lour.) Merrill. in mice and rats

• Published in: Pharmacology, biochemistry and behavior biochemistry and behavior, 2013-10, Vol.111, p.97-101
• Main Authors: Yang, Tao, Kong, Bin, Gu, Jian-Wen, Kuang, Yong-Qin, Cheng, Lin, Yang, Wen-Tao, Cheng, Jing-Min, Ma, Yuan, Yang, Xiao-Kun
• Format: Article
• Language: English
• Subjects: Animals; Anticonvulsant; Anticonvulsants - pharmacology; Blotting, Western; Brain - drug effects; Brain - enzymology; Brain - metabolism; Epilepsy; gamma-Aminobutyric Acid - metabolism; Glucosides - pharmacology; Glutamate Decarboxylase - metabolism; Glutamic Acid - metabolism; Heterocyclic Compounds, 2-Ring - pharmacology; Hypnotics and Sedatives - pharmacology; Male; Mice; Mice, Inbred ICR; Paederia scandens; Paederosidic acid; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Rubiaceae - chemistry; Sedative
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/j.pbb.2013.08.015

This study was designed to evaluate the anticonvulsant and sedative effects of paederosidic acid isolated from Paederia scandens (Lour.) Merrill. in mice and rats. In the present study, anticonvulsant activities of paederosidic acid were evaluated by maximal electroshock and pentylenetetrazole-induced seizures in male mice. Then, pentobarbital sodium-induced sleeping time and locomotor activity tests in mice were used to evaluate the sedative effects of paederosidic acid. Finally, the mechanism of paederosidic acid was explored by evaluating the contents of Glu and GABA in the brain, and Western blot was used to measure GAD65 expression in the mouse brain. Paederosidic acid (5, 10, 20, and 40mg/kg, ip) had significant anticonvulsant and sedative effects. Moreover, paederosidic acid increased brain gamma-aminobutyric acid and decreased glutamic acid in the brain, and it up-regulated expressions of GAD 65. In conclusion, our results suggest that paederosidic acid may be a promising future therapeutic agent for treatment of epilepsy. •We investigated anticonvulsant and sedative effects of paederosidic acid from Paederia scandens.•Treatment of paederosidic acid have significant anticonvulsant and sedative effects in mice.•The paederosidic acid can increase GABA and decrease Glu, and up-regulate expressions of GAD 65.