Digestibility of Transglutaminase Cross-Linked Caseinate versus Native Caseinate in an In Vitro Multicompartmental Model Simulating Young Child and Adult Gastrointestinal Conditions

Aim of this study was to investigate the digestion of transglutaminase cross-linked caseinate (XLC) versus native caseinate (NC) in solution and in cheese spread under digestive conditions for adults and children mimicked in a gastrointestinal model. Samples were collected for gel electrophoresis an...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-08, Vol.61 (31), p.7636-7644
Main Authors: Havenaar, Robert, de Jong, Aard, Koenen, Marjorie E, van Bilsen, Jolanda, Janssen, Armand M, Labij, Erik, Westerbeek, Hans J. M
Format: Article
Language:English
Subjects:
Adult
adults
Biological and medical sciences
caseinates
Caseins - chemistry
Caseins - metabolism
cheese spreads
children
crosslinking
digestibility
Digestion
Food industries
food matrix
Fundamental and applied biological sciences. Psychology
Gastrointestinal Tract - enzymology
Gastrointestinal Tract - metabolism
gel electrophoresis
Humans
Models, Biological
pepsin
peptides
protein-glutamine gamma-glutamyltransferase
simulation models
small intestine
stomach
Transglutaminases - metabolism
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Summary:Aim of this study was to investigate the digestion of transglutaminase cross-linked caseinate (XLC) versus native caseinate (NC) in solution and in cheese spread under digestive conditions for adults and children mimicked in a gastrointestinal model. Samples were collected for gel electrophoresis and nitrogen analysis. The results showed no relevant differences between XLC and NC for total and α-amino nitrogen in digested fraction under adult and child conditions. However, the rate of digestion was depending on the food matrix. Gel electrophoresis showed the gastric breakdown of XLC without formation of pepsin resistant peptides larger than 4 kDa. NC was slowly digested in the stomach with formation of pepsin resistant fragments and was still detectable in the stomach after 90 min. In the small intestine the proteins were rapidly digested. XLC was digested to small peptides, while NC was resistant against pepsin digestion under gastric conditions of adults and children.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf402824u