Recent advances in reactive oxygen species measurement in biological systems

•Reactive oxygen species (ROS) as biological signaling molecules.•The use of redox sensitive fluorescent probes for the measurement of ROS.•Genetic approaches for in vitro ROS measurement.•The use of novel nanoprobes from ROS detection in cells. Reactive oxygen species (ROS) play an essential role i...

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Bibliographic Details
Published in:Trends in biochemical sciences (Amsterdam. Regular ed.) 2013-11, Vol.38 (11), p.556-565
Main Authors: Woolley, J.F., Stanicka, J., Cotter, T.G.
Format: Article
Language:English
Subjects:
Animals
dynamics
fluorescence
fluorescent probes
Humans
hydrogen peroxide
Molecular Probes - chemistry
nanoparticles
Nanostructures
nanotubes
nitric oxide
reactive oxygen species
Reactive Oxygen Species - analysis
redox
ROS
ROS detection
ROS measurement
ROS probes
ROS signaling
Signal Transduction
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Summary:•Reactive oxygen species (ROS) as biological signaling molecules.•The use of redox sensitive fluorescent probes for the measurement of ROS.•Genetic approaches for in vitro ROS measurement.•The use of novel nanoprobes from ROS detection in cells. Reactive oxygen species (ROS) play an essential role in facilitating signal transduction processes within the cell. However, the precise details of the redox dynamics involved are not well understood. The generation of ROS is tightly controlled both spatially and temporally within the cell, making the study of ROS dynamics particularly difficult. In order to measure these dynamics, precise tools that can specifically examine the relevant ROS are required. Recent advancements in methodologies for ROS measurement have allowed the study of ROS biology at a level of precision previously unachievable. Here, we discuss improvements to fluorescent ROS dye technologies, genetically encoded ROS reporters, nanoparticle delivery systems, and nanotube ROS probes. These technologies improve specificity, localization and sensitivity over previously available ROS probes.
ISSN:0968-0004
1362-4326
DOI:10.1016/j.tibs.2013.08.009