Synthesis, characterization, and cytotoxicity of TMC-graft-poly(vinyl alcohol) copolymers

• Published in: Carbohydrate research 2013-11, Vol.381, p.153-160
• Main Authors: Martins, Alessandro F., Bueno, Pedro V.A., Follmann, Heveline D.M., Nocchi, Samara R., Nakamura, Celso V., Rubira, Adley F., Muniz, Edvani C.
• Format: Article
• Language: English
• Subjects: Cell Proliferation - drug effects; Cell Survival - drug effects; Chitosan; Chitosan - analogs & derivatives; Chitosan - chemical synthesis; Chitosan - chemistry; Chitosan - toxicity; composite polymers; Copolymers; cytotoxicity; Cytotoxins - chemical synthesis; Cytotoxins - chemistry; Cytotoxins - toxicity; Dose-Response Relationship, Drug; Graft; HCT116 Cells; Humans; Molecular Structure; N-trimethyl chitosan; nuclear magnetic resonance spectroscopy; Poly(vinyl alcohol); polyvinyl alcohol; Polyvinyls - chemical synthesis; Polyvinyls - chemistry; Polyvinyls - toxicity; scanning electron microscopy; Solubility; Structure-Activity Relationship; thermogravimetry; viability; Water - chemistry; X-radiation
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2012.11.014

Physical structure of the new copolymers based on N-trimethyl chitosan/poly(vinyl alcohol) (TMC-g-PVA) depicting the major interactions that occur among the segments of polymers chains of 6-O-succinate-N-trimethyl chitosan (STMC) and PVA. [Display omitted] • The high intensity of intermolecular interactions explained the elevated DG. • The copolymers presented low crystallinity in relation to pure PVA. • The copolymers showed lower cytotoxicity on the HCT-116 cells. N-trimethyl chitosan-graft-poly(vinyl alcohol) (TMC-g-PVA) copolymers were prepared. The grafting reactions were conducted in water changing the feed ratios of poly(vinyl alcohol)/6-O-succinate-N-trimethyl chitosan (PVA/STMC). The structure of TMC-g-PVA copolymers was characterized through 1H NMR spectroscopy, thermogravimetric analysis (TGA/DTG), wide-angle X-ray scattering (WAXS) and scanning electron microscopy (SEM). The quaternization degree (DQ) and substitution degree (DS) of N-trimethyl chitosan (TMC) and 6-O-succinate-N-trimethyl chitosan (STMC) were determined by 1H NMR, being the spectroscopy 14.0 and 5.5mol-% found, respectively. The viability of HCT-116 cancerous cells was investigated at different concentrations. The effect of PVA/STMC ratios on the cytotoxicity of the TMC-g-PVA copolymers was examined and the CC50 values determined for every case.