Three-dimensional structure of (Na super(+) + K super(+))-ATPase revealed by electron microscopy of two-dimensional crystals

Prolonged incubation of membrane fragments containing homogeneous (Na super(+) + K super(+))-ATPase with Mg super(2+), K super(+) and VO sub(3) at 4 degree C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 angstrom, b = 118 angstrom, gamma = 108 deg...

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Bibliographic Details
Published in:FEBS letters 1985-01, Vol.190 (1), p.73-76
Main Authors: Ovchinnikov, YuA, Demin, V V, Barnakov, AN, Kuzin, A P, Lunev, A V, Modyanov, N N, Dzhandzhugazyan, K N
Format: Article
Language:English
Subjects:
crystals
electron microscopy
membranes
quaternary structure
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Summary:Prolonged incubation of membrane fragments containing homogeneous (Na super(+) + K super(+))-ATPase with Mg super(2+), K super(+) and VO sub(3) at 4 degree C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 angstrom, b = 118 angstrom, gamma = 108 degree . The crystals correspond to the two-sided plane group p21. By combining tilted electronmicroscopic views of the crystals, a three-dimensi onal structure of (Na super(+) + K super(+))-ATPase was calculated at similar to 20 angstrom resolution. The unit cell is formed by two ( alpha beta )-subunits which are in contact in their central parts. The structure was compared with chemical modification and immunochemical data; the arrangement of intra-and extramembrane domains was proposed.
ISSN:0014-5793