Effect of phytic acid used as etchant on bond strength, smear layer, and pulpal cells

This study aimed to evaluate the effect of phytic acid (IP6), used as etchant, on resin–dentin bond strength, smear layer removal, and the viability of pulpal cells. Flat dentin surfaces with smear layer were etched with 1% IP6 for 60, 30, or 15 s; in the control group 37% phosphoric acid (PA) was u...

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Bibliographic Details
Published in:European journal of oral sciences 2013-10, Vol.121 (5), p.482-487
Main Authors: Nassar, Mohannad, Hiraishi, Noriko, Islam, Md. Sofiqul, Aizawa, Mamoru, Tamura, Yukihiko, Otsuki, Masayuki, Kasugai, Shohei, Ohya, Keiichi, Tagami, Junji
Format: Article
Language:English
Subjects:
Acid Etching, Dental - methods
Animals
bond strength
Cell Survival
Dental Bonding - methods
Dental Pulp - cytology
Dentin-Bonding Agents - chemistry
Dentistry
Humans
Microscopy, Electron, Scanning
Molar, Third
phosphoric acid
Phosphoric Acids - chemistry
phytic acid
Phytic Acid - chemistry
pulpal cells
Rats
Resin Cements - chemistry
Smear Layer
Surface Properties
Tensile Strength
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Summary:This study aimed to evaluate the effect of phytic acid (IP6), used as etchant, on resin–dentin bond strength, smear layer removal, and the viability of pulpal cells. Flat dentin surfaces with smear layer were etched with 1% IP6 for 60, 30, or 15 s; in the control group 37% phosphoric acid (PA) was used. Dentin surfaces were rinsed, blot‐dried, and bonded with an etch‐and‐rinse adhesive, followed by composite build‐ups. The specimens were subjected to tensile testing after 24 h of water storage at 37°C, and failure modes were determined using scanning electron microscopy. The effectiveness of IP6 to remove the smear layer was observed using scanning electron microscopy. To evaluate the effect on pulpal cells, solutions of 0.1 and 0.01% IP6 and of 3.7 and 0.37% PA were prepared and rat pulpal cells were treated with these solutions for 6 and 24 h. Cell viability was measured using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. The results demonstrated that all application times of IP6 produced bond‐strength values that were significantly higher than that of the control. Phytic acid effectively removed the smear layer and plugs, thus exposing the collagen network. Phytic acid had a minimal effect on pulpal cells, whereas PA resulted in a marked decrease in their viability.
ISSN:0909-8836
1600-0722
DOI:10.1111/eos.12064