Mouse monoclonal antibodies against paraoxon: potential reagents for immunoassay with constant immunochemical characteristics

Mice were immunized with (p-aminophenyl)paraoxon conjugated to protein by diazotization. The production and isolation of permanent hybridoma cell lines from the splenic lymphocytes of the immunized animals are described. The specificity of the two resultant paraoxon-specific monoclonal antibodies wa...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1985-11, Vol.33 (6), p.1237-1242
Main Authors: Brimfield, Alan A, Lenz, David E, Graham, Caroline, Hunter, Kenneth W
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical control
CHEMISTRY
CHIMIE
Control
Fundamental and applied biological sciences. Psychology
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
INSECTICIDAS
INSECTICIDE
INSECTICIDES
Methods and techniques. Experimental designs. Plant protection equipments
MICE
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
QUIMICA
RADIOIMMUNOASSAY
RATON
SOURIS
TECHNIQUE RADIO-IMMUNOLOGIQUE
TECNICA RADIOINMUNOLOGICA
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mice were immunized with (p-aminophenyl)paraoxon conjugated to protein by diazotization. The production and isolation of permanent hybridoma cell lines from the splenic lymphocytes of the immunized animals are described. The specificity of the two resultant paraoxon-specific monoclonal antibodies was tested with paraoxon analogues and seven insecticides. Significant cross reactivity was found with parathion and (p-aminophenyl)paraoxon, while no interfering compounds were found among the other insecticides tested. The affinities of the monoclonal antibodies were determined to be 9.4 x 10 super(4) and 1.7 x 10 super(5) L/mol. Specificity determinations proved the antibodies to be chemically unique.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00066a055