Does the genome of Corylus avellana L. contain sequences homologous to the self-incompatibility gene of Brassica?

Self-incompatibility is a genetic mechanism enforcing cross-pollination in plants. Hazelnut (Corylus avellana L.) expresses the sporophytic type of self-incompatibility, for which the molecular genetic basis is characterized only in Brassica. The hypothesis that the hazelnut genome contains homologs...

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Bibliographic Details
Published in:Theoretical and applied genetics 1996-10, Vol.93 (5-6), p.759-764
Main Authors: Hampson, C.R, Coleman, G.D, Azarenko, A.N. (Oregon State Univ., Corvallis (USA). Dept. of Horticulture)
Format: Article
Language:English
Subjects:
AUTOESTERILIDAD
AUTOSTERILITE
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
CORYLUS AVELLANA
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetics of eukaryotes. Biological and molecular evolution
GENOMAS
GENOME
GENOMES
GLICOPROTEINAS
GLYCOPROTEINE
GLYCOPROTEINS
NUCLEOTIDE SEQUENCE
Pteridophyta, spermatophyta
SECUENCIA NUCLEOTIDICA
SELF STERILITY
SEQUENCE NUCLEOTIDIQUE
Vegetals
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Summary:Self-incompatibility is a genetic mechanism enforcing cross-pollination in plants. Hazelnut (Corylus avellana L.) expresses the sporophytic type of self-incompatibility, for which the molecular genetic basis is characterized only in Brassica. The hypothesis that the hazelnut genome contains homologs of Brassica self-incompatibility genes was tested. The S-locus glycoprotein gene (SLG) and the kinase-encoding domain of the S-receptor kinase (SRK) gene of B. oleracea L. were used to probe blots of genomic DNA from six genotypes of hazelnut. Weak hybridization with the SLG probe was detected for all hazelnut genotypes tested; however, no hybridization was detected with PCR-generated probes corresponding to two conserved regions of the SLG gene. One of these PCR probes included the region of SLG encoding the 11 invariant cysteine residues that are an important structural feature of all S-family genes. The present evidence suggests that hazelnut DNA hybridizing to SLG differs significantly from the Brassica gene, and that the S-genes cloned from Brassica will not be useful for exploring self-incompatibility in hazelnut.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00224073