Prognostic significance of HIF-1a, CA-IX, and OPN in T1-T2 laryngeal carcinoma treated with radiotherapy

• Published in: The Laryngoscope 2013-09, Vol.123 (9), p.2154-2160
• Main Authors: Wachters, Jan E., Schrijvers, Michiel L., Slagter-Menkema, Lorian, Mastik, Mirjam, de Bock, Geertruida H., Langendijk, Johannes A., Kluin, Philip M., Schuuring, Ed, van der Laan, Bernard F. A. M., van der Wal, Jacqueline E.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, Neoplasm - metabolism; Biomarkers, Tumor - analysis; Carbonic Anhydrase IX; Carbonic Anhydrases - metabolism; Carcinoma, Squamous Cell - mortality; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - radiotherapy; Clinical outcomes; Cohort Studies; Confidence intervals; Databases, Factual; Disease-Free Survival; Female; head-and-neck squamous cell carcinoma; Humans; Hypoxia markers; Hypoxia-Inducible Factor 1, alpha Subunit - analysis; Immunohistochemistry; Kaplan-Meier Estimate; Laryngeal Neoplasms - mortality; Laryngeal Neoplasms - pathology; Laryngeal Neoplasms - radiotherapy; laryngeal squamous cell carcinoma; local control; Male; Middle Aged; Neoplasm Invasiveness - pathology; Neoplasm Staging; Osteopontin - metabolism; overall survival; Peptide Fragments - metabolism; Prognosis; Proportional Hazards Models; Radiation therapy; radiotherapy; Retrospective Studies; Risk Assessment; Survival Analysis; Treatment Outcome
• ISSN: 0023-852X; 1531-4995
• DOI: 10.1002/lary.23831

Objectives/Hypothesis To examine the prognostic value of hypoxia inducible factor HIF‐1a, CA‐IX, and OPN on clinical outcome in patients with T1–T2 supraglottic laryngeal squamous cell carcinoma (LSCC) treated with primarily radiotherapy (RT). Study Design Retrospective cohort study. Methods Tumor tissue sections of 60 patients with T1–T2 supraglottic LSCC treated with primarily radiotherapy were assessed immunohistochemically for expression of HIF‐1a, CA‐IX, and OPN. The relationship of protein expression and classical clinical parameters with clinical outcome was studied, using Cox regression and Kaplan‐Meier survival analyses. Results Neither HIF‐1a nor CA‐IX was of prognostic significance toward local control or overall survival in T1–T2 supraglottic LSCC. Cox regression survival analysis showed no relation between HIF‐1a or CA‐IX expression and local control (HR [hazard ratio] 1.07, CI [95% confidence interval] 0.29–3.87; HR 0.34, CI 0.04–2.58). Furthermore, OPN expression was not associated with local control (HR 1.37, CI 0.45–4.17) and overall survival (HR 0.99, CI 0.44–2.21). Our earlier findings in T1–T2 glottic LSCC (Schrijvers et al., 2008) could not be confirmed. Conclusion The absence of prognostic significance for HIF‐1a and CA‐IX toward local control in supraglottic LSCC, unlike glottic LSCC, suggests that supraglottic LSCC might represent another biological entity. Level of Evidence N/A. Laryngoscope, 123:2154–2160, 2013