Effect of DHA+EPA on oxidative stress and apoptosis induced by ischemia-reperfusion in rat kidneys

Apoptosis, as well as necrosis, has an important role in post‐ischemic renal pathology. The effect of pretreatment with Docosahexaenoic acid+Eicosapentaenoic acid (DHA+EPA) on renal injury and apoptotic protein expression was evaluated. Right nephrectomy was completed on male Wistar rats (255–300 g)...

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Published in:Fundamental & clinical pharmacology 2013-12, Vol.27 (6), p.593-602
Main Authors: Ajami, Marjan, Davoodi, Sayed Hossein, Habibey, Rouhollah, Namazi, Nasim, Soleimani, Mansoureh, Pazoki-Toroudi, Hamidreza
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - drug effects
Biological and medical sciences
Cardiology. Vascular system
Catalase - metabolism
Disease Models, Animal
Docosahexaenoic Acids - administration & dosage
Docosahexaenoic Acids - pharmacology
Eicosapentaenoic Acid - administration & dosage
Eicosapentaenoic Acid - pharmacology
Kidney - blood supply
Kidney - drug effects
Kidney - pathology
Kidney Function Tests
Male
Malondialdehyde - metabolism
Medical sciences
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Omega-3 fatty acids
Oxidative Stress - drug effects
Pharmacology. Drug treatments
Rats
Rats, Wistar
reactive oxygen species
renal ischemia reperfusion
Renovascular diseases
Reperfusion Injury - drug therapy
Reperfusion Injury - physiopathology
Superoxide Dismutase - metabolism
Time Factors
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Summary:Apoptosis, as well as necrosis, has an important role in post‐ischemic renal pathology. The effect of pretreatment with Docosahexaenoic acid+Eicosapentaenoic acid (DHA+EPA) on renal injury and apoptotic protein expression was evaluated. Right nephrectomy was completed on male Wistar rats (255–300 g). The rats received DHA+EPA (200 mg/kg/day) of distilled water orally for 14 days before ischemia reperfusion (IR) or sham operation. A total of 81 rats were divided into three main groups with 6, 24 and 48 h of post‐operation or reperfusion period. Serum creatinine (SCr), BUN, creatinine clearance (CCr) and fractional excretion of sodium (FENa) were measured. Tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities, Bax and Bcl‐2 protein expressions and renal histological injury were determined. SCr, BUN and FENa increased 6–48 h of reperfusion (P 
ISSN:0767-3981
1472-8206
DOI:10.1111/j.1472-8206.2012.01066.x