Metabolic Transit of Dietary Methylglyoxal

Methylglyoxal (MGO) is responsible for the pronounced antibacterial activity of manuka honey, in which it may reach concentrations up to 800 mg/kg. As MGO formed in vivo is discussed to play a role in diabetic complications, the metabolic transit of dietary MGO was studied within a 3 day dietary rec...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-10, Vol.61 (43), p.10253-10260
Main Authors: Degen, Julia, Vogel, Maria, Richter, Doreen, Hellwig, Michael, Henle, Thomas
Format: Article
Language:English
Subjects:
Adult
Digestion
Female
Gas Chromatography-Mass Spectrometry
Honey - analysis
Humans
Male
Molecular Structure
Pyruvaldehyde - chemistry
Pyruvaldehyde - metabolism
Pyruvaldehyde - urine
Young Adult
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Summary:Methylglyoxal (MGO) is responsible for the pronounced antibacterial activity of manuka honey, in which it may reach concentrations up to 800 mg/kg. As MGO formed in vivo is discussed to play a role in diabetic complications, the metabolic transit of dietary MGO was studied within a 3 day dietary recall with four healthy volunteers. Determination of MGO in 24 h urine was performed with GC-MS after derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine, and d-lactate was quantified enzymatically. Following a diet virtually free from MGO and other glycation compounds, a defined amount of MGO (500 μmol in manuka honey) was administered in the morning of day 2. Renal excretion was between 0.1 and 0.4 μmol/day for MGO and between 50 and 220 μmol/day for d-lactate. No influence on excretion of both compounds was observed following administration of MGO. To investigate the stability of MGO under physiological conditions, a simulated in vitro gastrointestinal digestion was performed with MGO-containing honey. After 8 h of in vitro digestion, only 5–20% of the initial methylglyoxal was recovered. This indicates that dietary MGO is rapidly degraded during the digestion process in the intestine and, therefore, exerts no influence on the MGO level in vivo.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf304946p