Determination, by fluorescein diacetate staining, of fungal viability during mycoparasitism

Trichoderma hamatum is a mycoparasite of Rhizoctonia solani. When stained with the vital stain fluorescein diacetate (FDA), both interacting fungi showed fluorescence during the first 24 h of incubation. Thereafter R. solani showed only a faint fluorescence while T. hamatum was still glowing up to 7...

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Bibliographic Details
Published in:Soil biology & biochemistry 1986, Vol.18 (3), p.315-319
Main Authors: Barak, Rina, Chet, Ilan
Format: Article
Language:English
Subjects:
Rhizoctonia solani
Trichoderma hamatum
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Summary:Trichoderma hamatum is a mycoparasite of Rhizoctonia solani. When stained with the vital stain fluorescein diacetate (FDA), both interacting fungi showed fluorescence during the first 24 h of incubation. Thereafter R. solani showed only a faint fluorescence while T. hamatum was still glowing up to 7 days. When each of these fungi were grown alone they showed a significant fluorescence for 4 days. This technique for determination of fungal viability during mycoparasitism may indicate that the initial attack occurs while the host is still alive.
ISSN:0038-0717
1879-3428
DOI:10.1016/0038-0717(86)90067-2