Isolation of mitochondrial DNA sequences that distinguish male-sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench

We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzyme-digested DNA of 28 sorghum lines repr...

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Bibliographic Details
Published in:Theoretical and applied genetics 1995-06, Vol.90 (7/8), p.1180-1187
Main Authors: Xu, G.W, Cui, Y.X, Schertz, K.F, Hart, G.E
Format: Article
Language:English
Subjects:
accessions
clones
cytoplasmic male sterility
DNA probes
genomics
genotype
isolation
lines
mitochondrial DNA
restriction fragment length polymorphism
Sorghum bicolor
Southern blotting
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Summary:We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzyme-digested DNA of 28 sorghum lines representing sources of different cytoplasmic male-sterility (CMS) groups. Four cytoplasmic types were defined on the basis of the pattern of DNA fragments detected. Similar analyses of 50 additional diverse sorghum accessions suggested that three of the four cytoplasmic types may be diagnostic for CMS. Also, three other cytoplasmic types were discovered. These and other mitochondrial DNA clones may be useful molecular tools for "fingerprinting" sterility-inducing cytoplasms in breeding programs, determining cytoplasmic diversity among germ plasm accessions, and identifying new sources of cytoplasm that induce male sterility.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00222941