Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L

Sixty-eight indica japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Oryza sativa L.) with four enzyme digestions (EcoR1, EcoR5, Hind3 and Dra1). Twenty-one DNA clones were isolated as indica japonica subspecies-differentiating probes. A se...

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Bibliographic Details
Published in:Theoretical and applied genetics 1995-05, Vol.90 (6), p.878-884
Main Authors: Qian, H.R, Zhuang, J.Y, Lin, H.X, Lu, J, Zheng, K.L. (China National Rice Research Inst., Hangzhou (China). Biotechnology Dept.)
Format: Article
Language:English
Subjects:
ADN
Agronomy. Soil science and plant productions
Biological and medical sciences
Classical and quantitative genetics
Classical and quantitative genetics. Population genetics. Molecular genetics
Classical genetics, quantitative genetics, hybrids
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics and breeding of economic plants
Genetics of eukaryotes. Biological and molecular evolution
IDENTIFICACION
IDENTIFICATION
ORYZA SATIVA
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SOUS ESPECE
SUBESPECIES
VARIEDADES
VARIETE
Vegetals
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Summary:Sixty-eight indica japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Oryza sativa L.) with four enzyme digestions (EcoR1, EcoR5, Hind3 and Dra1). Twenty-one DNA clones were isolated as indica japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00222026