Assessment of Extent of Apoptosis and DNA Defragmentation in Chilled Semen of Stallions During the Breeding Season

Contents The objective of the study was to assess apoptosis and DNA defragmentation in equine semen diluted and chilled to +4°C. Semen was collected from nine fertile stallions, including four Arabian thoroughbreds and five coldbloods. Examinations were carried out immediately after semen collection...

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Bibliographic Details
Published in:Reproduction in domestic animals 2013-10, Vol.48 (5), p.826-832
Main Authors: Krakowski, L, Obara, J, Wąchocka, A, Piech, T, Bartoszek, P, Kostro, K, Tatara, MR
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Apoptosis
Apoptosis - physiology
Breeding of animals
Cold Temperature
Deoxyribonucleic acid
DNA
DNA Fragmentation
Horses
Horses - physiology
Male
Semen - physiology
Semen Analysis - veterinary
Semen Preservation - veterinary
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Summary:Contents The objective of the study was to assess apoptosis and DNA defragmentation in equine semen diluted and chilled to +4°C. Semen was collected from nine fertile stallions, including four Arabian thoroughbreds and five coldbloods. Examinations were carried out immediately after semen collection (0) and at five storage times (24, 48, 72, 96 and 120 h). The basic semen evaluation was performed in terms of volume, sperm concentration, viable sperm percentage, progressive motility and morphology. Using flow cytometry, DNA defragmentation and cell membrane integrity of spermatozoa were determined. The results of basic tests did not demonstrate significant differences amongst stallions, except for progressive sperm motility, which was significantly higher (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12170