Recovery and purification of rapamycin from the culture broth of Mtcc 5681

In this study of the recovery and purification of rapamycin from the culture broth of an actinomycetes strain MTCC 5681, we investigated various factors such as biomass separation, suitable solvents for extraction, normal phase and flash chromatographic conditions and selective precipitation to obta...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2013-03, Vol.16 (5), p.219-225
Main Authors: Rani, Polavarapu Baby, Kumar, Battula Suneel, Rao, A K S Bhujanga, Sreenivasrao, S, Narasu, Mangamoori Lakshmi
Format: Article
Language:English
Subjects:
Actinobacteria - chemistry
Actinobacteria - growth & development
Actinobacteria - metabolism
Biomass
Chromatography - methods
Culture Media - chemistry
Culture Media - metabolism
Fermentation
Sirolimus - chemistry
Sirolimus - isolation & purification
Sirolimus - metabolism
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Summary:In this study of the recovery and purification of rapamycin from the culture broth of an actinomycetes strain MTCC 5681, we investigated various factors such as biomass separation, suitable solvents for extraction, normal phase and flash chromatographic conditions and selective precipitation to obtain rapamycin in substantially pure form of the product. Adsorption chromatography particularly with normal phase and flash chromatography, in combination with centrifugal decantation is found to be the most suitable for separation as well as purification of rapamycin. Centrifugal decantation technique is likely to emerge as an efficient, industrially scalable, high yielding and economical process for biomass separation. The purity of rapamycin obtained through the method described was 99.4% which has not been reported so far.
ISSN:1028-8880
DOI:10.3923/pjbs.2013.219.225