Variation in free-radical damage in rice cell suspensions with different embryogenic potentials

Levels of free-radical-mediated lipid peroxidation were monitored in cell-suspension cultures of Oryza sativa L. possessing different embryogenic potentials. Oxidative stress was evaluated using assays which sequentially assessed the stages of lipid peroxidation (diene conjugation, peroxidation, and...

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Bibliographic Details
Published in:Planta 1992-10, Vol.188 (3), p.296-305
Main Authors: Benson, E.E, Lynch, P.T, Jones, J
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Antioxidants
Biological and medical sciences
Biotechnology
catalase
Cell lines
cell suspension culture
embryo (plant)
embryo culture
embryogenesis
Embryonic cells
enzyme activity
Enzymes
Free radicals
Fundamental and applied biological sciences. Psychology
lipid peroxidation
Lipids
Oryza sativa
Oxidative stress
oxygen
peroxidase
Peroxides
Plant cells
Plant physiology and development
Rice
stress
superoxide dismutase
Tissue cultures, protoplasts
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Summary:Levels of free-radical-mediated lipid peroxidation were monitored in cell-suspension cultures of Oryza sativa L. possessing different embryogenic potentials. Oxidative stress was evaluated using assays which sequentially assessed the stages of lipid peroxidation (diene conjugation, peroxidation, and the formation of secondary lipid-peroxidation products). Lipid peroxidation was significantly higher in a cell line which had lost embryogenic ability compared with lines which still retained this capacity. Superoxide dismutase (EC 1.15.1.1) activity did not vary significantly between the embryogenic and previously embryogenic lines; however, catalase (EC 1.11.1.6) and peroxidase (EC 1.11.1.7) activities were significantly lower in the line which had lost embryogenic ability. Metabolic activity (as estimated by reduction of triphenyl tetrazolium chloride decreased with diminishing embryogenic potential and was especially low in cell lines which never exhibited embryogenic capabilities. The possible involvement of free radicals in the loss of embryogenic potential of rice cells is discussed.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00192795