Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench)

A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS...

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Bibliographic Details
Published in:Euphytica 2013-10, Vol.193 (3), p.383-390
Main Authors: Gao, Jianming, Xia, Buxian, Luo, Feng, Sun, Shoujun, Pei, Zhongyou, Gui, Zhi, Yuan, Qinghua, Li, Xianglin
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Behavior
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cytoplasm
Ethylenediaminetetraacetic acid
Fundamental and applied biological sciences. Psychology
Genes
Genetic markers
Genetic research
Genetics and breeding of economic plants
Grain
Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia
Life Sciences
Male sterility
Offspring
Plant breeding: fundamental aspects and methodology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Pollen
Povidone
Selective breeding
Sorghum
Sorghum bicolor
Sugar
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F 2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F 5 /BC 3 ) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future.
ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-013-0939-6