PCR testing can be as accurate as culture for diagnosis of Ichthyophonus hoferi in Yukon River Chinook salmon Oncorhynchus tshawytscha

We evaluated the comparability of culture and PCR tests for detecting Ichthyophonus in Yukon River Chinook salmon Oncorhynchus tshawytscha from field samples collected at 3 locations (Emmonak, Chena, and Salcha, Alaska, USA) in 2004, 2005, and 2006. Assuming diagnosis by culture as the 'true�...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2013-07, Vol.105 (1), p.21-25
Main Authors: Hamazaki, Toshihide, Kahler, Eryn, Borba, Bonnie M, Burton, Tamara
Format: Article
Language:English
Subjects:
Animals
Canada - epidemiology
Fish Diseases - parasitology
Freshwater
Ichthyophonus
Ichthyophonus hoferi
Mesomycetozoea - isolation & purification
Mesomycetozoea Infections - diagnosis
Mesomycetozoea Infections - epidemiology
Mesomycetozoea Infections - parasitology
Oncorhynchus tshawytscha
Rivers
Salmon
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Summary:We evaluated the comparability of culture and PCR tests for detecting Ichthyophonus in Yukon River Chinook salmon Oncorhynchus tshawytscha from field samples collected at 3 locations (Emmonak, Chena, and Salcha, Alaska, USA) in 2004, 2005, and 2006. Assuming diagnosis by culture as the 'true' infection status, we calculated the sensitivity (correctly identifying fish positive for Ichthyophonus), specificity (correctly identifying fish negative for Ichthyophonus), and accuracy (correctly identifying both positive and negative fish) of PCR. Regardless of sampling locations and years, sensitivity, specificity, and accuracy exceeded 90%. Estimates of infection prevalence by PCR were similar to those by culture, except for Salcha 2005, where prevalence by PCR was significantly higher than that by culture (p < 0.0001). These results show that the PCR test is comparable to the culture test for diagnosing Ichthyophonus infection.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao02606