Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M

Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective imm...

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Bibliographic Details
Published in:Journal of microbiology, immunology and infection immunology and infection, 2013-08, Vol.46 (4), p.253-258
Main Authors: Motaharinia, Yousef, Rezaee, Mohammad Ali, Rashidi, Ahmad, Jalili, Ali, Rezaie, Mohammad Jafar, Shapouri, Reza, Hossieni, Werya, Rahmani, Mohammad Reza
Format: Article
Language:English
Subjects:
Adjuvants
Adjuvants, Immunologic - administration & dosage
Alum
Alum Compounds - administration & dosage
Animals
Antibodies, Bacterial - blood
Bacterial Load
Brucella melitensis
Brucella melitensis - immunology
Brucella melitensis 16 M
Brucella Vaccine - administration & dosage
Brucella Vaccine - immunology
Brucellosis - immunology
Brucellosis - prevention & control
Disease Models, Animal
Immunoglobulin G - blood
Infectious Disease
Interferon-gamma - blood
Interleukin-4 - blood
Male
Medical Education
Mice
Mice, Inbred BALB C
Naloxone
Naloxone - administration & dosage
Protective immunity
Serum - immunology
Serum - microbiology
Survival Analysis
Vaccination - methods
Vaccines, Inactivated - administration & dosage
Vaccines, Inactivated - immunology
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Summary:Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB). Methods Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-γ and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. Results The serum levels of IFN-γ, IL-4, and immunoglobulin G in the NLX + alum + HKB group were shown to be significantly increased ( p  
ISSN:1684-1182
1995-9133
DOI:10.1016/j.jmii.2012.03.011