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Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M
Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective imm...
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| Published in: | Journal of microbiology, immunology and infection immunology and infection, 2013-08, Vol.46 (4), p.253-258 |
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| container_end_page | 258 |
| container_issue | 4 |
| container_start_page | 253 |
| container_title | Journal of microbiology, immunology and infection |
| container_volume | 46 |
| creator | Motaharinia, Yousef Rezaee, Mohammad Ali Rashidi, Ahmad Jalili, Ali Rezaie, Mohammad Jafar Shapouri, Reza Hossieni, Werya Rahmani, Mohammad Reza |
| description | Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB). Methods Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-γ and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. Results The serum levels of IFN-γ, IL-4, and immunoglobulin G in the NLX + alum + HKB group were shown to be significantly increased ( p |
| doi_str_mv | 10.1016/j.jmii.2012.03.011 |
| format | article |
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Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB). Methods Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-γ and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. Results The serum levels of IFN-γ, IL-4, and immunoglobulin G in the NLX + alum + HKB group were shown to be significantly increased ( p < 0.05). Furthermore, the lowest bacterial load was observed in this group. The survival rate in groups vaccinated with combinations containing adjuvants was 100%. Conclusion The combination of NLX and alum enhanced the immunogenicity of HKB, which can be used in the vaccination of animals and humans at risk of the disease.</description><identifier>ISSN: 1684-1182</identifier><identifier>EISSN: 1995-9133</identifier><identifier>DOI: 10.1016/j.jmii.2012.03.011</identifier><identifier>PMID: 22727892</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject><![CDATA[Adjuvants ; Adjuvants, Immunologic - administration & dosage ; Alum ; Alum Compounds - administration & dosage ; Animals ; Antibodies, Bacterial - blood ; Bacterial Load ; Brucella melitensis ; Brucella melitensis - immunology ; Brucella melitensis 16 M ; Brucella Vaccine - administration & dosage ; Brucella Vaccine - immunology ; Brucellosis - immunology ; Brucellosis - prevention & control ; Disease Models, Animal ; Immunoglobulin G - blood ; Infectious Disease ; Interferon-gamma - blood ; Interleukin-4 - blood ; Male ; Medical Education ; Mice ; Mice, Inbred BALB C ; Naloxone ; Naloxone - administration & dosage ; Protective immunity ; Serum - immunology ; Serum - microbiology ; Survival Analysis ; Vaccination - methods ; Vaccines, Inactivated - administration & dosage ; Vaccines, Inactivated - immunology]]></subject><ispartof>Journal of microbiology, immunology and infection, 2013-08, Vol.46 (4), p.253-258</ispartof><rights>2012</rights><rights>Copyright © 2012. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-329701efaea8eaff043bb6540817b3199dfd83cda182e868ce32d538dffcccc3</citedby><cites>FETCH-LOGICAL-c444t-329701efaea8eaff043bb6540817b3199dfd83cda182e868ce32d538dffcccc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22727892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Motaharinia, Yousef</creatorcontrib><creatorcontrib>Rezaee, Mohammad Ali</creatorcontrib><creatorcontrib>Rashidi, Ahmad</creatorcontrib><creatorcontrib>Jalili, Ali</creatorcontrib><creatorcontrib>Rezaie, Mohammad Jafar</creatorcontrib><creatorcontrib>Shapouri, Reza</creatorcontrib><creatorcontrib>Hossieni, Werya</creatorcontrib><creatorcontrib>Rahmani, Mohammad Reza</creatorcontrib><title>Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M</title><title>Journal of microbiology, immunology and infection</title><addtitle>J Microbiol Immunol Infect</addtitle><description>Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB). Methods Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-γ and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. Results The serum levels of IFN-γ, IL-4, and immunoglobulin G in the NLX + alum + HKB group were shown to be significantly increased ( p < 0.05). Furthermore, the lowest bacterial load was observed in this group. The survival rate in groups vaccinated with combinations containing adjuvants was 100%. Conclusion The combination of NLX and alum enhanced the immunogenicity of HKB, which can be used in the vaccination of animals and humans at risk of the disease.</description><subject>Adjuvants</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Alum</subject><subject>Alum Compounds - administration & dosage</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacterial Load</subject><subject>Brucella melitensis</subject><subject>Brucella melitensis - immunology</subject><subject>Brucella melitensis 16 M</subject><subject>Brucella Vaccine - administration & dosage</subject><subject>Brucella Vaccine - immunology</subject><subject>Brucellosis - immunology</subject><subject>Brucellosis - prevention & control</subject><subject>Disease Models, Animal</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious Disease</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin-4 - blood</subject><subject>Male</subject><subject>Medical Education</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Naloxone</subject><subject>Naloxone - administration & dosage</subject><subject>Protective immunity</subject><subject>Serum - immunology</subject><subject>Serum - microbiology</subject><subject>Survival Analysis</subject><subject>Vaccination - methods</subject><subject>Vaccines, Inactivated - administration & dosage</subject><subject>Vaccines, Inactivated - immunology</subject><issn>1684-1182</issn><issn>1995-9133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNUslu1TAUjRCIlsIPsEBesiDBQ14GCSGViqFSEQu6txz7ht5XDyVOHrxf4Wu56WtZsEB44UnnHPuec4viueCV4KJ5va22AbGSXMiKq4oL8aA4Fn2_KXuh1EPaN11dCtHJo-JJzlvOayU3zePiSMpWtl0vj4tf59EtdsYUWRrZzZRmoNMOGIawRJz3zHwzGPPMhmmx4H3KmBlGFtACG_ZsZ6zFaG4VfuB8xQyzKQz3VyQajU8_U4RXzPgl0BwduwIzl9foPTj27iBsWACPM8T1AdGwz0-LR6PxGZ7drSfF5Yf3l2efyosvH8_PTi9KW9f1XCrZt1zAaMB0YMaRihyGZlPzTrSDIjvc6DplnSEfoGs6C0q6jercOFoa6qR4eZCl4r8vkGcdMN9-KEJashZ13UnJybD_gMq2JXjDCSoPUDulnCcY9c2EwUx7Lbhe09Nbvaan1_Q0V5rSI9KLO_1lCOD-UO7jIsCbAwDIjx3CpLNFiBYcTpSbdgn_rf_2L7r1GNEafw17yNu0TBQW1aEzcfTXtX_W9hGSU-_0rfoN3pTC5w</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Motaharinia, Yousef</creator><creator>Rezaee, Mohammad Ali</creator><creator>Rashidi, Ahmad</creator><creator>Jalili, Ali</creator><creator>Rezaie, Mohammad Jafar</creator><creator>Shapouri, Reza</creator><creator>Hossieni, Werya</creator><creator>Rahmani, Mohammad Reza</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20130801</creationdate><title>Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M</title><author>Motaharinia, Yousef ; Rezaee, Mohammad Ali ; Rashidi, Ahmad ; Jalili, Ali ; Rezaie, Mohammad Jafar ; Shapouri, Reza ; Hossieni, Werya ; Rahmani, Mohammad Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-329701efaea8eaff043bb6540817b3199dfd83cda182e868ce32d538dffcccc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adjuvants</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Alum</topic><topic>Alum Compounds - administration & dosage</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacterial Load</topic><topic>Brucella melitensis</topic><topic>Brucella melitensis - immunology</topic><topic>Brucella melitensis 16 M</topic><topic>Brucella Vaccine - administration & dosage</topic><topic>Brucella Vaccine - immunology</topic><topic>Brucellosis - immunology</topic><topic>Brucellosis - prevention & control</topic><topic>Disease Models, Animal</topic><topic>Immunoglobulin G - blood</topic><topic>Infectious Disease</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin-4 - blood</topic><topic>Male</topic><topic>Medical Education</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Naloxone</topic><topic>Naloxone - administration & dosage</topic><topic>Protective immunity</topic><topic>Serum - immunology</topic><topic>Serum - microbiology</topic><topic>Survival Analysis</topic><topic>Vaccination - methods</topic><topic>Vaccines, Inactivated - administration & dosage</topic><topic>Vaccines, Inactivated - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Motaharinia, Yousef</creatorcontrib><creatorcontrib>Rezaee, Mohammad Ali</creatorcontrib><creatorcontrib>Rashidi, Ahmad</creatorcontrib><creatorcontrib>Jalili, Ali</creatorcontrib><creatorcontrib>Rezaie, Mohammad Jafar</creatorcontrib><creatorcontrib>Shapouri, Reza</creatorcontrib><creatorcontrib>Hossieni, Werya</creatorcontrib><creatorcontrib>Rahmani, Mohammad Reza</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of microbiology, immunology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Motaharinia, Yousef</au><au>Rezaee, Mohammad Ali</au><au>Rashidi, Ahmad</au><au>Jalili, Ali</au><au>Rezaie, Mohammad Jafar</au><au>Shapouri, Reza</au><au>Hossieni, Werya</au><au>Rahmani, Mohammad Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M</atitle><jtitle>Journal of microbiology, immunology and infection</jtitle><addtitle>J Microbiol Immunol Infect</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>46</volume><issue>4</issue><spage>253</spage><epage>258</epage><pages>253-258</pages><issn>1684-1182</issn><eissn>1995-9133</eissn><abstract>Background/Purpose A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella . Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB). Methods Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-γ and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. Results The serum levels of IFN-γ, IL-4, and immunoglobulin G in the NLX + alum + HKB group were shown to be significantly increased ( p < 0.05). Furthermore, the lowest bacterial load was observed in this group. The survival rate in groups vaccinated with combinations containing adjuvants was 100%. Conclusion The combination of NLX and alum enhanced the immunogenicity of HKB, which can be used in the vaccination of animals and humans at risk of the disease.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>22727892</pmid><doi>10.1016/j.jmii.2012.03.011</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of microbiology, immunology and infection, 2013-08, Vol.46 (4), p.253-258 |
| issn | 1684-1182 1995-9133 |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
| subjects | Adjuvants Adjuvants, Immunologic - administration & dosage Alum Alum Compounds - administration & dosage Animals Antibodies, Bacterial - blood Bacterial Load Brucella melitensis Brucella melitensis - immunology Brucella melitensis 16 M Brucella Vaccine - administration & dosage Brucella Vaccine - immunology Brucellosis - immunology Brucellosis - prevention & control Disease Models, Animal Immunoglobulin G - blood Infectious Disease Interferon-gamma - blood Interleukin-4 - blood Male Medical Education Mice Mice, Inbred BALB C Naloxone Naloxone - administration & dosage Protective immunity Serum - immunology Serum - microbiology Survival Analysis Vaccination - methods Vaccines, Inactivated - administration & dosage Vaccines, Inactivated - immunology |
| title | Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M |
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