In vitro studies on intestinal peptide transport in horses

Published data on the physiology of nutrient transport across the equine intestine are limited, and the existence and relevance of peptide transporters are still unknown in the horse. In the present study, the equine intestinal peptide transport was investigated by Ussing chamber experiments using t...

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Bibliographic Details
Published in:Journal of animal science 2013-11, Vol.91 (11), p.5220-5228
Main Authors: Cehak, A, Schröder, B, Feige, K, Breves, G
Format: Article
Language:English
Subjects:
Alkaline Phosphatase
Animals
Biological Transport - physiology
Carrier Proteins - physiology
Dipeptides - metabolism
Female
Horses - physiology
Jejunum - physiology
Male
Microvilli - metabolism
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Summary:Published data on the physiology of nutrient transport across the equine intestine are limited, and the existence and relevance of peptide transporters are still unknown in the horse. In the present study, the equine intestinal peptide transport was investigated by Ussing chamber experiments using the radioisotope tracer technique and by uptake studies into brush border membrane vesicles (BBMV). Jejunal mucosae of 16 healthy adult horses were used. Tissue samples were mounted in Ussing chambers, and electrophysiological parameters as well as unidirectional flux rates of the radiolabelled dipeptide glycylglutamine (Gly-Gln) were determined. The short-circuit current (Isc) response to the luminal addition of Gly-Gln was significantly greater compared to the Isc response to glycylsarcosine (Gly-Sar) addition (P
ISSN:1525-3163
DOI:10.2527/jas.2013-6325