Liver regeneration following partial hepatectomy is improved by enhancing the HGF/Met axis and Akt and Erk pathways after low-power laser irradiation in rats

• Published in: Lasers in medical science 2013-11, Vol.28 (6), p.1511-1517
• Main Authors: Araújo, Tiago G., de Oliveira, Alexandre G., Tobar, Natália, Saad, Mario J. Abdalla, Moreira, Luciana R., Reis, Edmyr R., Nicola, Ester M. D., de Jorge, Gracinda L., dos Tártaro, Rodolfo R., Boin, Ilka F. S. F., Teixeira, Antonio R. Franchi
• Format: Article
• Language: English
• Subjects: Abdominal surgery; Animals; Cell growth; Dentistry; Hepatectomy; Hepatocyte Growth Factor - metabolism; Ki-67 Antigen - metabolism; Lasers; Liver diseases; Liver Regeneration - physiology; Liver Regeneration - radiation effects; Low-Level Light Therapy; Male; MAP Kinase Signaling System - radiation effects; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Phosphorylation; Photonics; Proliferating Cell Nuclear Antigen - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Proto-Oncogene Proteins c-met - metabolism; Quantum Optics; Rats; Rats, Wistar; Rodents; Signal Transduction - radiation effects
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-013-1264-y

A simple, easy, and safe procedure aiming to improve liver regeneration could be of great clinical benefit in critical situations such as major hepatectomy, trauma, or hemorrhage. Low-power laser irradiation (LPLI) has come into a wide range of use in clinical practice by inducing regeneration in healthy and injured tissues. However, the effect of LPLI on the process of liver regeneration, especially those related to the molecular mechanisms, is not fully understood. Thus, the aim of the present study was to investigate the main molecular mechanisms involved in liver regeneration of partially hepatectomized rats exposed to LPLI. We used Wistar male rats, which had their remaining liver irradiated or not with LPLI (wavelength of 632.8 nm and fluence of 65 mW/cm 2 ) for 15 min after a 70 % hepatectomy. We subsequently investigated hepatocyte growth factor (HGF), Met, Akt, and Erk 1/2 signaling pathways through protein expression and phosphorylation analyses along with cell proliferation (proliferating cell nuclear antigen (PCNA) and Ki-67) using immunoblotting and histological studies. Our results show that LPLI can improve liver regeneration as shown by increased HGF protein expression and the phosphorylation levels of Met, Akt, and Erk 1/2 accompanied by higher levels of the PCNA and Ki-67 protein in the remnant livers. In summary, our results suggest that LPLI may play a clinical role as a simple, fast, and easy-to-perform strategy in order to enhance the liver regenerative capacity of a small liver remnant after hepatectomy.