Ultrastructural and electron energy loss spectroscopy studies of sequestration mechanisms of Cd and Cu in the marine diatom Skeletonema costatum

The marine diatom Skeletonema costatum was used to study mechanisms of detoxification when submitted to cadmium and copper contamination. After 96 h of growth, concentration corresponding to 50% growth inhibition (IC50, 96 h) was 0.224 mg/L for cadmium and 0.045 mg/L for copper, indicating that copp...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1997-08, Vol.33 (2), p.147-155
Main Authors: NASSIRI, Y, MANSOT, J. L, WERY, J, GINSBURGER-VOGEL, T, AMIARD, J. C
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Cadmium
Cadmium - analysis
Copper
Copper - analysis
Detoxification
Diatoms - chemistry
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on plants and fungi
Electrons
Energy loss
Fundamental and applied biological sciences. Psychology
Microscopy, Electron
Spectrophotometry, Atomic
Spectroscopy
Spectrum analysis
Sulfur
Trace metals
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Summary:The marine diatom Skeletonema costatum was used to study mechanisms of detoxification when submitted to cadmium and copper contamination. After 96 h of growth, concentration corresponding to 50% growth inhibition (IC50, 96 h) was 0.224 mg/L for cadmium and 0.045 mg/L for copper, indicating that copper is more toxic for S. costatum than cadmium. Heavy cellular damages were observed for cadmium and copper concentrations close to the IC50. Exposure to these concentrations induced a migration of inclusions from the peripheral cytoplasm to the vacuole. Electron energy loss spectroscopy (EELS) investigations demonstrated that Cd and Cu were specifically trapped in these inclusions. However, Cu was less sequestered than cadmium in the vacuole. EELS determination of oxidation states evidenced that trace metals were sequestered as Cd2+ and Cu2+. Nitrogen and sulfur are involved in metallic storage, especially in the case of cadmium contamination.
ISSN:0090-4341
1432-0703
DOI:10.1007/s002449900236