Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography

Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high‐speed counter‐current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2013-09, Vol.36 (18), p.3123-3129
Main Authors: Yue, Hui-Lan, Zhao, Xiao-Hui, Mei, Li-Juan, Shao, Yun
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography
Columns (structural)
Countercurrent Distribution
General pharmacology
Glycosides
Glycosides - chemistry
Glycosides - isolation & purification
High speed
HSCCC
Lamiaceae - chemistry
Lamiophlomis rotata (Benth.) Kudo
Macroporous resins
Medical sciences
Molecular chemistry
NMR spectroscopy
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phenylpropanoid glycosides
Phenylpropionates - chemistry
Phenylpropionates - isolation & purification
Polymers
Porosity
Resins
Resins, Synthetic - chemistry
Separation
Surface Properties
Traditional Chinese medicines
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high‐speed counter‐current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two‐phase solvent system composed of ethyl acetate/n‐butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27 mg of forsythoside B, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside B with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one‐step separation within 4 h from 150 mg of crude extract. The recoveries of the five PhGs after MR‐HSCCC separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. The chemical structures of all five compounds were identified by 1H and 13C NMR spectroscopy.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201300268