Cancer Diagnostics: A Trachea-Inspired Bifurcated Microfilter Capturing Viable Circulating Tumor Cells via Altered Biophysical Properties as Measured by Atomic Force Microscopy (Small 18/2013)

In isolating circulating tumor cells (CTCs), which exist in extremely low concentrations in the blood, current methods have been limited by a trade-off between recovery rate and purity. To overcome this challenge, M. S. Kim, S. S. Lee, J.-G. Lee, and co-workers present a sized-based filtration metho...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2013-09, Vol.9 (18), p.3005-3005
Main Authors: Kim, Minseok S., Kim, Jinhoon, Lee, Wonho, Cho, Sang-Joon, Oh, Jin-Mi, Lee, June-Young, Baek, Sanghyun, Kim, Yeon Jeong, Sim, Tae Seok, Lee, Hun Joo, Jung, Goo-Eun, Kim, Seung-Il, Park, Jong-Myeon, Oh, Jin Ho, Gurel, Ogan, Lee, Soo Suk, Lee, Jeong-Gun
Format: Article
Language:English
Subjects:
Bifurcations
biophysics
Blood
cancer diagnostics
Cellular
Circulating
circulating tumor cells
filters
microfluidics
Microorganisms
Nanotechnology
Purity
Recovery
Tumors
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Summary:In isolating circulating tumor cells (CTCs), which exist in extremely low concentrations in the blood, current methods have been limited by a trade-off between recovery rate and purity. To overcome this challenge, M. S. Kim, S. S. Lee, J.-G. Lee, and co-workers present a sized-based filtration method that combines two strategies: (1) using specific binding of solid microbeads conjugated with anti-EpCAM antibodies to enhance the discrimination of CTCs from other blood constituents (mainly white blood cells) along with (2) a novel bio-inspired bifurcated microfilter that minimizes fluidic stresses on these cells, once captured. The method approaches maximal recovery rate and purity, while allowing viable CTCs to be isolated, enabling not only their enumeration but also further molecular and cellular evaluation.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201370105