Effect of Huanglianjiedu Tang on fever in rats induced by 2, 4-dinitrophenol

OBJECTIVE： To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol （DNP） and the effect of Huanglianjiedu Tang （HLJDT） on the fever. METHODS： The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spe...

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Bibliographic Details
Published in:Journal of traditional Chinese medicine 2013-08, Vol.33 (4), p.492-499
Main Authors: Liu, Shumin, Wang, Na, Chen, Pingping, Li, Xuzhao, Liu, Changfeng
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Biomarkers - urine
Chromatography, High Pressure Liquid
Dinitrophenols - adverse effects
Drugs, Chinese Herbal - administration & dosage
Fever - drug therapy
Fever - etiology
Fever - urine
Humans
Male
Metabolomics
Rats
Rats, Wistar
Tandem Mass Spectrometry
主要成分分析
二硝基酚
发热
大鼠
氨基酸代谢
生物标志物
超高效液相色谱
黄连解毒汤
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Summary:OBJECTIVE： To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol （DNP） and the effect of Huanglianjiedu Tang （HLJDT） on the fever. METHODS： The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry （UPLC/ESI-Q-TOF-MS） at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis. RESULTS： Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hy-droxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS.These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively. CONCLUSION： HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.
ISSN:0255-2922
DOI:10.1016/S0254-6272(13)60154-5