Hydroxyapatite/Collagen Bone-Like Nanocomposite

Our group has succeeded to synthesize material with bone-like nanostructure and bone-like inorganic and organic composition via self-organization mechanism between them using simultaneous titration method under controlled pH and temperature. The hydroxyapatite/collagen (HAp/Col) bone-like nanocompos...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2013/11/01, Vol.36(11), pp.1666-1669
Main Author: Kikuchi, Masanori
Format: Article
Language:English
Subjects:
Animals
biological reaction
Bone and Bones
Bone Marrow Cells - cytology
chemical composition
collagen
Collagen - chemistry
Dental Implants
Durapatite - chemistry
Humans
hydroxyapatite
nanocomposite
Nanocomposites - chemistry
nanostructure
Osteoclasts - cytology
Titanium - chemistry
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Summary:Our group has succeeded to synthesize material with bone-like nanostructure and bone-like inorganic and organic composition via self-organization mechanism between them using simultaneous titration method under controlled pH and temperature. The hydroxyapatite/collagen (HAp/Col) bone-like nanocomposite completely incorporated into bone remodeling process to be substituted by new bone. Cells cultured on the HAp/Col revealed very interesting reactions. Osteoblast-like MG63 cells showed upregulation of alkaline phosphatase >3 times greater than MG63 cells cultured on tissue culture polystyrene (TCPS). MG63 cells 3-dimensionally cultured in a “HAp/Col sponge,” a porous HAp/Col having sponge-like viscoelasticity, accumulated calcium phosphate nodules on extracellular matrices they secreted. Bone marrow cells co-cultured with osteoblasts on HAp/Col differentiated to osteoclasts without differentiation supplements. This phenomenon is not found in cells cultured on hydroxyapatite ceramics and TCPS, and rarely in cells cultured on dentin. These results suggest that HAp/Col is a good candidate for tissue engineering of bone as well as bone filler. In a clinical test as a bone filler, the HAp/Col sponge was significantly better than porous β-tricalcium phosphate. The HAp/Col sponge has been approved by the Japanese government and will be used as greatly needed bone filler in patients. In addition to the above, HAp/Col coating on titanium revealed higher osteoconductivity than HAp-coated titanium and bare titanium and improved direct bonding between titanium and newly formed bone. The HAp/Col coating may be used for metal devices requiring osseointegration.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b13-00460